Amandine PASQUIER

En résumé

Entreprises

• Novimmune - Bioanalytical Development Assistant

  2016 - maintenant Work in a lab with a team of experienced assistants to support bioanalytical development and testing for various activities such as manufacturing process development, drug substance/drug product characterization and stability studies.
  
  Activities:
  - Organize and execute routine testing following existing QA documents,
  - Interpret and communicate testing results while respecting timelines,
  - Participate to lab maintenance activities: lab stock management (including re-ordering), common buffer/solution preparations, and routine equipment servicing,
  - Support bioanalytical method development and/or qualification if needed,
  - Draft and validation of QA documentation (deviations, SOPs, etc.),
  - Prepare presentations and communicate data during internal meetings,
  - Contribute to improvements for the lab organization/activities,
  - Study Monitor (Scientific contact) for bioanalytics outsourced to GMP CROs for lot release and stability studies: reviewed the documentation such as task orders, protocols, etc; assured the accuracy, completeness and time delivery of the data packages,
  - Performed audit of external GMP CROs (as co-auditor).
  
  Technical skills: Cell culture, ELISA development or routine testing, FACS based assay, gene reporter assay.
  
  Bioanalytical Development Technician II: September 2016 to August 2017
  Bioanalytical Development Assistant: September 2017 to today

• Inserm - Research Engineer (Internship)

  PARIS 13 2014 - 2016 Team: Retroviral Oncogenesis - Pr MAHIEUX R.
  
  Projects:
  i) How to control HTLV-1 associated disease: preventing de novo infection using antiviral therapy.
  ii) Detection and quantification of STLV-1 and SFV proviral load in blood and saliva of naturally infected non-human primate.
  iii) Crystallization: production and purification of p65/RelA protein.
  
  Technical skills: i) cell culture (T-lymphocytes cell lines infected or virus free, human embryonic kidney 293 cells), virus purification, screening of antiviral therapy in vitro, transfection, electroporation. ii) Annual sanitary control of baboons (Papio papio), Polymerase chain reaction (PCR): Nested PCR, qPCR, GoTaq PCR, electrophoresis, DNA cloning. iii) Western Blot, p19 sandwich ELISA, bacterial protein synthesis.
  
  Responsibilities: UV area (used by 2 INSERM units), upgrade of liquid nitrogen tank management and organization.

• Tolerys Biolabs - Research and Development Technician (Apprenticeship)

  2013 - 2014 Project: Development and application of assays from mycobacteria-derived preparation in the field of chronic inflammatory diseases or oncology.
  
  Technical skills : XTT Cell Viability Assay, myeloperoxidase activity assay, Cytokine sandwich ELISA, Quantification of FAP protein by competitive ELISA (2 plates assay). Responsibilities: Participate to lab maintenance activities: lab stock management (including re-ordering), common buffer/solution preparations, routine equipment servicing.

• Centre de recherche en cancérologie de Lyon (CRCL) - Research and Development Technician (Internship)

  2012 - 2012 Team: Targeting of the tumor and its immune environment – Ms. DUTOUR A.
  
  Project: Inhibitor of the PI3K/Akt/mTOR pathway: new treatment option for chondrosarcoma?
  
  Technical skills: Western Blot, Immunohistochemistry, Immunofluorescence.
  

• Centre de recherche en cancérologie de Lyon (CRCL) - Research and Development Technician (Internship)

  2012 - 2012 Team: Targeting of the tumor and its immune environment – Ms. DUTOUR A.
  
  Project: Inhibitor of the PI3K/Akt/mTOR pathway: new treatment option for chondrosarcoma?
  
  Technical skills: Western Blot, Immunohistochemistry, Immunofluorescence.

Formations

• Ecole Pratique Des Hautes Etudes

  Paris 2014 - 2016 Diplôme Sciences de la Vie et de la Terre
  
  Academic supervision: Pr Exbrayat Jean-Marie.
  
  Obtention of 'Certificat de Capacité à la Recherche (CCR)' in July 2015 (end of the first year).
  
  Lesson follow the first year:
  - Flow Cytometry (DS: 14/20),
  - Retrovirology (DS: 18/20 ; DM : 16/20) et
  - Normal and pathologic development of immune system (DS: 17,8/20).
  
  Lesson follow the second year:
  - Cellular biology - Improvement (DS : 17,5/20)

• Université Catholique De Lyon

  Lyon 2013 - 2014 Titre d'Assistant de laboratoire biologie, biochimie

• Lycée Jean Baptiste De La Salle

  Lyon 2011 - 2013 BTS Bioanalyses et contrôles