Mes compétences :
Microscopy
X-rays
Microbiology
Cell Culture
Biotechnology
Entreprises
Institut Pasteur
- PhD candidate
Paris2013 - 2016Driving a research project on the understanding of the natural bacterial transformation mechanism in Streptococcus pneumoniae:
• Optimization of the expression and purification of two proteins with unknown activities
• Establishment and implementation of experiment using molecular biology (PCR, cloning, mutation in vivo, double bacterial hybrid) and biophysical techniques (electron microscopy, X-ray crystallography)
• Establishment of a collaboration with Harvard University group and a group of mass spectrometry at the Pasteur Institute
• Presentation informal account of each month at the thesis supervisor and oral presentation at international workshop
• Supervision of a bachelor student from the "Amgen Scholars Program" during 2 months
Johns Hopkins Bloomberg School of Public Health
- Visiting scholar
2012 - 2013Structural study of two proteins involved in the multiplication of malaria in the human body:
• Expression and purification of soluble proteins
• Continued crystallization of the most promising and optimization of crystal growth
• Participation in the determination of the structure at 2.5 Å resolution of a protease of Plasmodium falciparum
• Monthly presentation of the results in English to the team
• Writing an internship report
• Machines used: Äkta for protein purification (SEC, affinity, ion exchange), Mosquito Nano Drop Setter
IPL
- Laboratory Technician,
2011 - 2011Establishment of a quality approach on a technic of water analysis:
• Determination of the concentration of nitrogen in wasterwater in routine use Kjeldahl method.
• Daily interpretation of the results to send it to the head of department.
• Independent choice of the samples of interest to determine the uncertainty of the method.
• Presentation of the report on robustness of the approach end of the internship.
Complétude
- Private Tutoring
Paris2010 - 2012Tutoring biology, chemistry, mathematics and physics to more than 20 high school students:
• Preparation of exercises and custom exams.
• Teachings of concepts not covered during absences.
• Following most students for more than 2 years.
Continuation of this activity outside "Complétude" since the beginning of the thesis.
CNRS
- Research Assistant
Paris2009 - 2012Laboratory of "Environnements et Paléoenvironnements Océaniques et Continentaux" at the University of Bordeaux - 50 hours per month over 11 months
Determining the rate of sedimentation in marine samples:
• Preparation and autonomous classification of samples
• Making gamma spectrometry experiments by measuring 210Pb and 226Ra
• Management of refilling the detectors with liquid nitrogen
• Analysis of the spectrum for sample dating
• Presentation of Excel sheet with the results to the supervisor twice monthly
Reladed courses:
• Programming classes (Python).
• Workshop on 3D cryo-EM solutions in 2015 - Maastricht : Conference on the analysis of individual particles, tomography and cryo-CLEM, practical work on sample preparation, image acquisition and 3D reconstruction via tomography.
• The 6th Brazil School for Single Particle cryo-EM: Learning of theoretical mathematics and physics for 3D structure
ENSTBB (Ecole Nationale Supérieure De Technologie Des Biomolécules De Bordeaux) (Bordeaux)
Bordeaux2011 - 2013Master's Degree Biotechnology
• Biotechnology and Bioproduction: genetic engineering, Cell culture, immunology.
• Engineering: mathematics, X-ray crystalloraphy, RMN, biophysics, downstream processes.
• Business Administration: English, Intellectual Property, GLP and GMP, companies management.
