Amy DIALLO

En résumé

Entreprises

• Institut Pasteur - PhD candidate

  Paris 2013 - 2016 Driving a research project on the understanding of the natural bacterial transformation mechanism in Streptococcus pneumoniae:
  
  • Optimization of the expression and purification of two proteins with unknown activities
  • Establishment and implementation of experiment using molecular biology (PCR, cloning, mutation in vivo, double bacterial hybrid) and biophysical techniques (electron microscopy, X-ray crystallography)
  • Establishment of a collaboration with Harvard University group and a group of mass spectrometry at the Pasteur Institute
  • Presentation informal account of each month at the thesis supervisor and oral presentation at international workshop
  • Supervision of a bachelor student from the "Amgen Scholars Program" during 2 months

• Johns Hopkins Bloomberg School of Public Health - Visiting scholar

  2012 - 2013 Structural study of two proteins involved in the multiplication of malaria in the human body:
  
  • Expression and purification of soluble proteins
  • Continued crystallization of the most promising and optimization of crystal growth
  • Participation in the determination of the structure at 2.5 Å resolution of a protease of Plasmodium falciparum
  • Monthly presentation of the results in English to the team
  • Writing an internship report
  • Machines used: Äkta for protein purification (SEC, affinity, ion exchange), Mosquito Nano Drop Setter

• IPL - Laboratory Technician,

  2011 - 2011 Establishment of a quality approach on a technic of water analysis:
  • Determination of the concentration of nitrogen in wasterwater in routine use Kjeldahl method.
  • Daily interpretation of the results to send it to the head of department.
  • Independent choice of the samples of interest to determine the uncertainty of the method.
  • Presentation of the report on robustness of the approach end of the internship.

• Complétude - Private Tutoring

  Paris 2010 - 2012 Tutoring biology, chemistry, mathematics and physics to more than 20 high school students:
  • Preparation of exercises and custom exams.
  • Teachings of concepts not covered during absences.
  • Following most students for more than 2 years.
  
  Continuation of this activity outside "Complétude" since the beginning of the thesis.

• CNRS - Research Assistant

  Paris 2009 - 2012 Laboratory of "Environnements et Paléoenvironnements Océaniques et Continentaux" at the University of Bordeaux - 50 hours per month over 11 months
  
  Determining the rate of sedimentation in marine samples:
  • Preparation and autonomous classification of samples
  • Making gamma spectrometry experiments by measuring 210Pb and 226Ra
  • Management of refilling the detectors with liquid nitrogen
  • Analysis of the spectrum for sample dating
  • Presentation of Excel sheet with the results to the supervisor twice monthly
  

Formations

• Université Pierre Et Marie Curie Paris 6 (UPMC)

  Paris 2013 - 2016 Doctor of Philosophy (PhD
  
  Reladed courses:
  • Programming classes (Python).
  • Workshop on 3D cryo-EM solutions in 2015 - Maastricht : Conference on the analysis of individual particles, tomography and cryo-CLEM, practical work on sample preparation, image acquisition and 3D reconstruction via tomography.
  • The 6th Brazil School for Single Particle cryo-EM: Learning of theoretical mathematics and physics for 3D structure

• ENSTBB (Ecole Nationale Supérieure De Technologie Des Biomolécules De Bordeaux) (Bordeaux)

  Bordeaux 2011 - 2013 Master's Degree Biotechnology
  
  • Biotechnology and Bioproduction: genetic engineering, Cell culture, immunology.
  • Engineering: mathematics, X-ray crystalloraphy, RMN, biophysics, downstream processes.
  • Business Administration: English, Intellectual Property, GLP and GMP, companies management.

• Lycée Michel Montaigne

  Bordeaux 2008 - 2010 Preparatory school to national exams of Engineering school
  
  • Biology
  • Sciences of earth
  • Physics
  • Chemistry
  • Mathematics

Réseau

Pas de contact professionnel