Charlotte HENRIET

En résumé

Entreprises

• Inra - PhD Student

  Paris 2015 - 2019 Key words : Sulfur nutrition, Drought, Seed quality, Leaf remobilization, Pea, System biology
  
  My PhD project aims to determine the impact of drought combined with sulfur deficiency on the development and quality of pea seeds, as well as foliar remobilization.
  
  The pea plants were produced on the Dijon high-throughput phenotyping platform (4PMI) and then transcriptomics, proteomics, metabolomics and ionomics analyzes were performed in partnership with external platforms.
  
  This project allowed the accumulation of valuable fundamental knowledge and the determination of a quality marker for the protein composition of pea seeds, an important factor for animal nutrition and seed utilization in industry.
  
  Supervisor : Dr Karine GALLARDO and Dr Vanessa VERNOUD
  
  - Implementation & Follow-up of experimental studies
  - Team management
  - Production & Analysis of data with R software
  - Supervision of trainees
  - Substitute Lecturer & Teaching assistant
  - Writing Articles, Posters & Protocols
  - Oral presentation of results at National & International conferences

• INRA - Project Engineer

  Paris 2015 - 2015 Key words : Nitrogen nutrition, Arabidopsis thaliana, Molecular biology
  
  My internship research project aimed to studying the nuclear export of transcription factor NLP7, a key regulator of nitrogen metabolism.
  
  Supervisor : Dr Anne KRAPP
  
  - DNA & RNA extraction
  - PCR & Quantitative reverse transcription PCR (RT-qPCR)
  - Oligonucleotide & Primer design
  - Seedlings & Cultured in vitro, greenhouse
  - Production of protoplasts & Transformation for transient expression
  - Confocal microscopy
  - Image processing with GIMP 2 and ImageJ

• INRA - Project Engineer

  Paris 2014 - 2014 Key words : Drought, Maize, Proteomics
  
  My internship research project aimed to studying the effect of transcription factor ZmMYB31 overexpression on leaf proteome and cell wall constituents in maize under contrasting water conditions.
  
  Supervisor : Dr Sylvie COURSOL
  
  - Protein extraction
  - Shotgun label-free quantitative proteomics in collaboration with the Proteomics Plateform Paris Sud-Ouest (PAPPSO)
  - FT-IR (Fourier transform infrared spectroscopy) analysis

Formations

• Université De Bourgogne

  Dijon 2015 - 2018 Doctor of Philosophy (PhD)
  
  Main Courses :
  
  - Fundamentals of management
  - Project management
  - Team management
  - Industrial & Intellectual property law
  - Introduction to consultancy cervices
  - Statistical analysis of data
  - Initiation to R
  - Summer school « SPS Summer School 2016 : From gene expression to genomic network »
  
  Secretary of the EDIFICE association (2016)
  Head of the scientific commission for the organization

• Université Paris 7 Denis Diderot

  Paris 2013 - 2015 Master's degree
  
  Known as Master in Plant Science since 2016.
  
  This master is a formation of two years completely oriented towards specific issues of science and crop production. It Is the result of the combination of three higher education institutions: the University Paris Diderot, the University of Evry Val d'Essonne and AgroParisTech engineering school. This specialization, coupled with good general knowledge

• Université Paris 7 Denis Diderot

  Paris 2010 - 2013 Bachelor's degree
  
  Main Courses :
  
  - Molecular biology & Biotechnologies
  - Genetic
  - Plant responses to the environment
  - Metabolic physiology

• Lycée Evariste Galois

  Sartrouville 2007 - 2010 Baccalauréat (French secondary school diploma)
  
  Associate Student at Board of Directors, Security Council & Disciplinary Board

Réseau

• Amandine DERQUENNES

• Coline DEBROSSE

• Guillaume CHANTREAU

• Iris GROSJEAN