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Diana ORDONEZ

MARSEILLE

En résumé

Mes compétences :
Flow Cytometry
intracellular signaling analysis
development of naturally occurring regulatory T ce
cytometric analysis
Western Blotting
T cell development
Immunology

Entreprises

  • Centre d'Immunologie de Marseille Luminy - Ingenieur de Recherche

    maintenant
  • Centre d'Immunologie de Marseille-Luminy - Ingenieur de Recherche

    2009 - maintenant Post-doctoral Position. Laboratory of ``Genetic dissection of the
    function of T cells and dendritic cells'' led by Drs. Bernard and
    Marie MALISSEN.

    Project: ``Characterization of neutropenic mouse model generated by random
    mutagenesis''

    * Multiparameter flow cytometry (18 parameters).

    * Immunophenotyping of hematopoietic cell types, including T cells, B cells,
    dendritic cells, natural killer cells (NK), mast cells, macrophages, monocytes
    and progenitors cells.

    * Cell sorting of populations poorly represented: mast cells, plasmocytoid
    dendritic cells, B1a and B1b cells.

    * Evaluation of neutrophil biology in steady state and inflammatory conditions:
    developmental stages, cell morphology, expression of transcripts coding for
    granule proteins and functional activity.

    * Collaborative participation with external laboratories to analyze the adaptive
    immune response in mice chronically infected with Brucella sp. and
    neutrophil requirement in NK cell development and activation.
  • Universidad Nacional Autónoma de México - PhD Student

    2004 - 2009 Laboratory of ``T cell development and activation''
    led by Dr. Gloria SOLDEVILA.

    Thesis subject: ``Role of CD5 in thymocyte development and regulatory T cell
    generation''

    * Phenotype and intracellular signaling analysis of regulatory T cells isolated
    from CD5 deficient mice. Detection of phospho-proteins by intracellular
    staining.

    * Biochemical characterization of CD5 cytoplasmic domain by
    immunoprecipitaton and western blot.

Formations

  • Pontificia Universidad Javeriana (Bogota)

    Bogota 1998 - 2003 Bachelors Degree

    Microbiologie

Réseau

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