PARIS 82012 - maintenantProject: Thorough identification of Shiga-toxin producing Escherichia coli (STEC) human pathogen found in Belgium.
* Development and validation of SYBR®Green qPCR assays to detect STEC in food matrices ;
* Development and validation of a Luminex system for thorough characterisation of genes present in detected strains to evaluate their pathogenicity. ;
UCL
- PhD student
2009 - 2014Project: Development of a system for detection and identification of foodborne pathogenic bacteria according to a ``Combinatory qPCR'' technology. (see publications)
* Development and validation of SYBR®Green qPCR assays to detect and discriminate Listeria spp. and Salmonella spp. in food matrices. (see publications)
* Evaluation of viability-qPCR using Propidium Monoazide (PMA). (see publications)
* Validation on swab samples of the developed CoSYPS Path Food system. (see publications) ;
IPH
- Junior Scientist
PARIS 82007 - 2009Projects: GMODetec and Co-Extra
* : Development and validation of SYBR®Green qPCR assays for detection of GM-events in food and feed products (see publications)
* Constuction of plasmid containing the amplicons of the CoSYPS assays and validation of their use as plasmid mix for positive control. Management of the CoSYPS Interlaboratory study (see publications).
* Study of the best storage conditions of the GMO feed matrix (flour, pellets and liquids) in order to be able to get valuable counter-expertise analysis.
* ISP collaboration project: Coaching/Teaching of colleagues from the Virology team in order to produce their own RNAm reference material thanks to plasmid transcription. ;
INRA-URGV
- Research Assistant
2006 - 2007: TILLING on EMS-mutagenised tomato collection.
Development of target genes screening strategies, detection of targeted genes mutants in the tomato population (studied targets: genes involved in DET1 complexe, virus resistance, plant architecture, ethylene and carotenoid pathway), identification of mutation's effect on the protein with bioinformatic tools.
INRA-URGV
- Research Assistant
2005 - 2006: Production and improvement of ENDO 1: an endonuclease enzyme used in TILLING technology (Targeted Induced Local Lesions In Genome), (see publications).
Production of ENDO 1, optimization of its conservation and activity. Charge of interface between URGV holder of ENDO1 patent and SERIAL GENETICS holder of the commercial licence.
D.I.A.S.
- Master Internship
2004 - 2004Project: Virus-Induced Gene Silencing used as a functional genomics tool in legumes using pea as a model plant.
* Host range study of the Pea Early Browning Virus using Agrobacterium-inoculation of a modified virus vector carrying the uidA reporter gene.
* Study of the importance of insert size with respect to virus symptoms versus silencing efficiency by constructing plasmids carrying different sizes of the marker gene (PDS) fused with different sizes of a control gene.
* The development of a protocol for seed Agrobacterium-inoculation. ;
