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Jean-Stéphane GATOT

LIÈGE

En résumé

I am a bio-chemist engineer and I have accomplished a PhD in agronomical sciences and bioengineering.

I conducted scientific research on various pathologies (leukemia, chronic diseases, inflammation, diabetes, AIDS), which allowed me to accumulate experience in project management and to develop many new testing methods.

In November 2010, I joined the QC department of a big pharmaceutical company, where I gained experience in compliance and validation of analytical methods for viral vaccines. Within this GMP and constantly evolving environment, I learned tools for project management and the philosophy of continuous improvement.

These professional experiences in R&D and QC have allowed me
- to gain skills in training of technicians or coaching of supervisors,
- to apply my skills in scientific analysis and technical writing (test procedures, validation protocols and reports, assessments, deviation reports, qualification protocols and reports),
- and to acquire a broad operational expertise in the following area: cell culture, virology, biology, biochemistry, epigenetics, bioassays, methods validation.

My analytical abilities are my greatest strength. I like to analyze large amount of scientific data and I can be synthetic while maintaining the important details. I have good listening skills. I like working in teams but I can also work individually. I am a worker persevering, methodical, organized, and I get fully involved in my job because I like the work well-done.

I am looking for a job that would allow me to use my skills and my experience to contribute to the success of the business by managing projects, completing missions or improving activities. I am motivated to work in a multidisciplinary environment, to share my knowledge, to contribute to innovation and optimization. I want to continue to learn, to discover, to improve myself and to develop myself professionally.

I can take part in the achievement of your projects in R&D, QC or QA or I can work as scientific analyst - technical writer.

Interested in my profile? Feel free to contact me on my mobile phone (+32 498175874) or by e-mail (jsgatot@voo.be).


Mes compétences :
Training
Project management
Qualité
Validation de méthodes
Process improvement
Analyse scientifique
Développement de méthodes
Rédaction technique
recherche et développement
Cell culture
Biotechnology
Continuous improvement
Immunology
Biochemistry
Quality
Research and development
Life sciences
Molecular biology
Biology

Entreprises

  • University Hospital of Liège, Department of Genetics - Temporary Senior Scientist

    2013 - maintenant Development of CGH (comparative genomic hybridization)-array for prenatal diagnostics

  • Available for new challenges - Looking for an exciting new job opportunity

    2012 - maintenant

  • GSK Vaccines - Manager Contrôle Qualité

    2010 - 2012 Main responsibilities :
    - QC Virology process validation and development
    - Reviewing/approval of technical protocols and reports (test procedures, validation protocols and reports, scientific assessments, deviation reports, qualification protocols and reports)
    - Validation of QC lab activities to ensure analytical testing compliance
    - Support of analytical life cycle

    Main achievements :
    - Reviewed and approved qualification protocols and reports for cleaning agents and thermal decontamination against viral vaccines
    - Reviewed, approved or wrote validation protocols and reports for continuous improvement of QC analytical methods
    - Managed the reorganization of QC Biology offices for optimization of the workspace
    - Led the improvement project for meetings quality within QC Biology
    - Improved the processes with internal customers (monitoring deadlines, project lifecycle, project sheet – order form, way of working)
    - Wrote the request for procedure change to replace primary monkey kidney cells by a continuous cell line for viral effective inactivation testing
    - Reviewed and validated technically QC Virology V-SD deviations
    - Coordinated periodic revalidation assessments for QC Virology methods in compliance with ICHQ2

    Key learnings : project management, lean culture, continuous improvement, methods validation

  • Université Libre de Bruxelles - Chercheur senior

    Bruxelles 2007 - 2010 Main responsibilities :
    - Evaluation of the potentials of novel histone desacetylase inhibitors to reactivate HIV-1 expression in latently infected cells (in collaboration with Tibotec, Mechelen, Belgium)
    - Identification of new combinations of compounds to decrease the pool of latently-infected reservoirs in patients treated with Highly Active Anti-Retroviral Therapy (HAART)

    Main achievements :
    - Developed testing procedures to evaluate the effect of new drugs to reactivate HIV-1 expression in latently infected cells (optimization of cellular concentration to test rare cells, cytotoxicity assay, purification of resting T CD4 cells using magnetic cell sorting, cellular population analysis using flow cytometry,...)
    - Initiated researches showing that histone methyltransferase inhibitors induce HIV-1 recovery in resting CD4+ T cells from HIV-1-infected HAART-treated patients
    - Recruited, trained and developed 1 trainee student, 1 PhD student and 2 technicians
    - Wrote applications for projects funding

    Key learnings : scientific project management, methods development, people management, project writing and budgeting, people development

  • Université Libre de Bruxelles - Chercheur senior

    Bruxelles 2006 - 2007 Main responsibility :
    Management of the project “Development of a tolerogenic vaccine against type I autoimmune diabetes”

    Main achievements :
    - Expressed and purified recombinant proteins (GST-foxp3, IGF2-thrombospondin,…)
    - Improved the purity of GST-foxp3 protein
    - Evaluated different methods to best transfect T cells and dendritic cells
    - Trained and supervised 1 traineeship student and 2 technicians

    Key learnings : scientific project management, methods development, people development

  • Université de Liège - Chercheur

    2002 - 2006 Main responsibilities :
    - Identification of new biomarkers in inflammatory diseases by a proteomic approach
    - Study of TANK/I-TRAF (TRAF family member associated NF-B activator / TRAF-interacting protein) -dependent NF-B pathways

    Main achievements :
    - Isolated 3 chronic obstructive pulmonary disease (COPD)-specific biomarkers
    - Identified new interaction partners for TANK using a yeast two hybrid approach
    - Cloned and expressed recombinant proteins
    - Demonstrated a new transduction pathway upon LPS-mediated IRF stimulation
    - Developed methods to highlight interactions between proteins (solubilization of membrane proteins by heating, solubilization of bacterial proteins by sonication, kinase assay,…)

    Key learnings : scientific project management, methods development

  • Fonds national belge de la recherche scientifique - Chercheur post-doctoral

    1999 - 2002 Main responsibilities :
    - Evaluation of chemokine receptors as receptor for bovine leukemia virus (BLV)
    - Study of bovine leukemia virus surface protein interactions with metal ions

    Main achievements :
    - Demonstrated the interaction of BLV and HTLV-1 SU protein with zinc
    - Performed site-directed mutagenesis of envelope proteins and viruses
    - Cloned and expressed recombinant envelope proteins
    - Developed methods to show interactions between envelope proteins and metal ions (ELISA test with envelope peptides, affinity chromatography,…)
    - Predicted the three-dimensional structure of the receptor-binding domain of the BLV gp51 protein using hydrophobic cluster analysis
    - Trained and supervised 2 traineeship students

    Key learnings : scientific project management, methods development, scientific writing

Formations

  • Gembloux Agro-Bio Tech (Gembloux)

    Gembloux 1994 - 1999 PhD

    sciences agronomiques et ingénierie biologique

  • Gembloux Agro Bio Tech-ULg (Gembloux)

    Gembloux 1989 - 1994 bioingénieur

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