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Johanna Sophie ALFEN

TOULOUSE

En résumé

Mes compétences :
Méthodiques en immunologie
Biologie cellulaire
Biochimie
Expérimentation animale
Biologie Moléculaire
Essais functionals cellulaires
Cytométrie en flux
Culture cellulaire

Entreprises

  • Instituto Nazionale Genetico e Moleculare (INGM) - Reseacher, PhD Student

    2011 - 2015 Thèse du Doctorat:
    ”Human in vivo occurring Tr1 cells: role and therapeutical potential in inflammatory bowel disease”

    Investigation of the importance of a regulatory T-cell subset in chronic diseases of the human intestine: Specimens of the human gut were processed and lymphocytes were analysed according to their phenotypical and functional characteristics. To test whether this particular regulatory T-cell subset could be of use in cell therapy cell-lines and clones were generated and examined over time.
  • clinique dermatologique du CHU de Freiburg - Thèse expérimentale du diplôme

    2009 - 2010 “The role of chemokines and dentritic cells in the process of homing-receptor imprinting on T cells”
    In the context of allergic contact dermatitis skin-specific and intestine-specific components and their role in the imprinting of homing receptors on inflammatory T-cells were investigated. For this purpose murine DC were loaded with tissue specific chemokines/substances and co-cultured with T-cells in vitro and changes in receptor expression were revealed by flow-cytometry. To assess the impact in vivo the mouse model of contact hypersensitivity (CHS) was used
  • Haus Landwasser - Surveillance nocturne

    2009 - 2010 Etablissement de réhabilitation psychiatrique
  • Max Planck Institute of Immunobiology - Assistante de recherche

    2008 - 2008 Assistance d'une post-doc, exécution d'expériences et travaux de laboratoire
  • Albert-Ludwigs-Universität Freiburg - Assistante de recherche

    2007 - 2008 Département de sciences humaines et sociales: Gender Studies
    Organisation d'un symposium international, Assistance générale, analyse et enregistrement d’article de recherche
  • Albert-Ludwigs-Universität Freiburg - Assistante à l'animalerie

    Paris 2006 - 2006
  • Pharmacia GmbH - Assistante

    2002 - 2004 Enregistrement des observations biologiques, maintenance des bases de données et travaux administratifs

Formations

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