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Inserm U1051
- Researcher in Vision Science
2017 - maintenant
Currently I am a postdoctoral researcher in the Vision Team, in the Inherited Optic Neuropathies and Mitochondrial Disorders Group. My subject is still the Wolfram Syndrome, its pathophysiology and gene therapy against the ensuing optic neuropathy. I continue to work with two mouse models, phenotyping them and treating the diseases with adapted gene therapies.
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Inserm
- Researcher PhD student
PARIS 13
2013 - 2016
Researching application of mouse models and appling gene therapy to develop a treatment against Wolfram Syndrome.
Team: Vision. Inherited Optic Neuropathies and Mitochondrial Disorders
Subject: Wolfram Syndrome: Pathophysiology and gene therapy of the optic neuropathy
Focus:
- Phenotyping of mouse models: - visual acuity (optomotor reflex)
- retinal ganglion cell function (pSTR, ERG)
- eye physiology (OCT, eye fundus)
- optic nerve loss (TEM)
- Neurological tests: - rotarod
- open field
- vestibular tests
- catwalk
- Gene therapy: intravitral and sub-retinal
Publications:
- Bonnet Wersinger D et al. Impairment of visual function and retinal ER stress activation in
Wfs1-deficient mice. PLoS One. 2014
- Angebault C et al. Endoplasmic reticulum protein WFS1 interacts with WIP1 to regulate mitochondrial functionality. Submitted
Funding: Labex EpiGenMed, Retina France, Fondation de France, Association de Syndrôme Wolfram, Fondation Recherche Médicale, AFM Téléthon
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Inserm
- Trainee
PARIS 13
2013 - 2013
Team: Molecular mechanisms of myelination/demyelination in peripheral nerves
Subject: Physiology and dynamics of mitochondria in peripheral nerves
Focus: Schwann cell and neuron transduction in the sciatic nerve
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Artimmune
- Volunteer Research Assistant
2012 - 2013
Local society for preclinical research
Focus:
- Bronchoalvolar lavage
- ELISA
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CNRS
- Trainee
Paris
2012 - 2013
Team: Allergy and lung inflammation
Subject: Role of IL-22 in the ozone-induced acute lung inflammation
Focus:
- Murine model of acute ozone-induced lung inflammation
- Pletysmography with Methacholine: invasive and non-invasive
- Bronchoalveolar lavage
- Training students
Funding: Socrates-Erasmus Exchange
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CNRS
- Trainee
Paris
2012 - 2012
IGMM
Team: Transcriptional control of angiogenesis
Subject: Role of LYL1 transcription factor in endothelial cell activation
Focus: Silencing LYL-1 gene in HUVEC cells by several types of magnetofection
Achievements: Poster presentation
Funding: Socrates-Erasmus Internship
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Jagiellonian University
- Research Assistant
2011 - 2012
Team: Medical Biotechnology
Subject: US microbubble-facilitated gene therapy against hindlimb ischemia
Focus:
- Pre-emptive delivery of hHO-1 and hVEGF under HRE promoter to ischemic limbs
- Plasmid amplification, technique establishment
- Grade of reperfusion, inflammation, angiogenesis, arteriogenesis and cell proliferation after hindlimb ischemia in HO-1 WT and KO mice
Techniques:
- in vivo: hind limb ischemia murine model, IVIS Lumina (D-luciferin), Laser Doppler
- in vitro: skeletal muscles and serum analysis: histology (H&E, isolectin-B4, α-SMA), proteins (ELISA, WB), mRNA (qPCR)
Achievements:
- MSc thesis in Biotechnology.
- Poster presentation (Edinburgh 2012)
- Publication: Jazwa A et al, Pre-emptive hypoxia-regulated HO-1 gene therapy improves the post-ischemic limb perfusion and tissue regeneration in mice. Cardiovasc Res. 2013
Funding: Homing Plus, Foundation for Polish Science
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Jagiellonian University
- Research Volunteer
2011 - 2011
Team: Medical Biotechnology
Subject: Microvascular progenitors. New model of tumor angiogenesis.
Focus:
- Isolation & characterization of murine BM-derived microvascular progenitor cells: FACS analysis
- New model of in vivo tumor angiogenesis with spheroid cell cultures
Achievements:
- Poster presentations (Kraków 2011, Edinburgh 2012)
Cooperation: Jagiellonian Centre for Experimental Therapeutics
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Jagiellonian University
- Research Volunteer
2010 - 2011
Team: Medical Biotechnology
Subject: Type I diabetes: HO-1 in hindlimb ischemia and wound healing.
- Cytoprotective and proangiogenic gene therapy for critical limb ischemia in diabetic mice
- Endothelial progenitor cells in hind limb ischemia therapy in diabetic mice
Focus:
- Mouse models of wound healing with silicone rings, and hindlimb ischemia
- Necropsy, immunohistology of muscle tissue, qualitative and quantitative analysis of proteins and nucleic acids → state of inflammation and re-vascularization.
Achievements:
- Contributed to 1 PhD thesis and 1 MSc thesis.
- Paper and poster presentations (Kraków 2011, Kraków 2011)
- Grochot-Przeczek A et al, Heme oxygenase-1 is required for angiogenic function of bone marrow-derived progenitor cells: role in therapeutic revascularization. Antioxid Redox Signal. 2014
Funding: The Welcome Trust - The Sunset Award, Ministry of Science and Higher Education
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Jagiellonian University
- Research Volunteer
2008 - 2010
Team: Medical Biotechnology
Subject: Anti-angiogenic therapies in cancer.
Focus:
- Effect of combretastatin-based compounds on neovasculogenesis in mimicked neoplastic habitat in vitro
- Basic methods of genetic engineering with viral transduction, 2D & 3D-models of EC cultures
- Mammalian cell cultures, proliferation and cell viability assays, mechanisms of angiogenesis
Achievements:
- BSc Thesis
Cooperation: ADAMED pharmaceutical company
