Karl SOBCZYK

En résumé

Entreprises

• Nektar Therapeutics -  Research Associate III

  2014 - 2017 -Developed and validated biochemical, cell, and immuno assays to support characterization of novel small molecules and biologics in diverse therapeutic areas.
  -Conducted routine screening assays, performed investigative studies on the mechanism of action of novel compounds, and
  interpreted and communicated experimental findings to project teams.
  -Worked on a wide range of cellular, biochemical, and immuno assays, performed mammalian cell culture, transfections,
  tissue preps, flow cytometry, FLIPR, MSD, and radioligand receptor binding assays.
  -Performed a small library screen and determined functional properties of hits.
  -Led the complete re-organization of the Controlled Drug Substance compound library and cataloging system.
  -Actively participated in the development of the In Vitro group database, and later the process of selecting a new ELN and
  database software provider.
  -Additional roles taken as lab manager, safety committee member, CDS manager, radioactive safety representative.

• Bayer - Research Scientist

  Lyon 2014 - 2014 -Provided method development, testing, and validation under minimal supervision of a more senior personnel. Project
  consisted of developing an analytical technique utilizing high-throughput capillary electrophoresis.

• Novartis - Associate Scientist II

  RUEIL MALMAISON 2012 - 2014 -Developed biochemical assays and supported on-going drug discovery projects. Assay methodologies included AlphaScreen,
  differential scanning fluorimetry, fluorescence polarization, and KinaseGlo.
  -Performed medium-throughput 384 well plate format assays utilizing multichannel micropipettes, standard liquid handling
  systems (Combi/FX Robot), and plate reader systems (Perkin Elmer EnVision, Tecan, AB ViiA7).
  -Developed assays for high-throughput screening projects. Determined conditions for optimal assay performance, including
  Michaelis-Menten kinetic analysis (kcat, Km).
  -Performed and analyzed thermodynamic properties using Surface Plasma Resonance (Biacore) and ITC.
  -Collected and analyzed scientific data and documented results in lab notebook.
  -Managed compound and assay plate ordering using internal software and published data to IT infrastructure.
  -Troubleshot experiments, wrote protocols, and prepared SOPs.
  -Presented data at departmental meetings.

• Harvard Medical School - Mass. General Hospital - Research Associate II

  2008 - 2010 -Studied the mechanisms of proteasome-mediated protein degradation via fluorescent enzymatic assays.
  -Designed, conducted, and analyzed enzymatic assays in 96- and 384-well plate format.
  -Main project consisted of developing and conducting a high throughput small molecule drug screen.
  -Operated and supervised multi-unit plate loaders, readers, pin transfers, and other machines.
  -Independently screened 230,000+ compounds and determined functional properties of hits.
  -Coded a multi-step Excel macro to calculate, analyze, and manage hits based on inputted thresholds.
  -Purified protein from E.Coli and mammalian stock tissue using different chromatography techniques.
  -After training and certification, worked with radioactive material for analytical protein degradation assays.
  -Routinely confirmed results via gel electrophoresis, ATPase, Bradford, and other qualitative/quantitative assays.

Formations

• Illinois Institute Of Technology (Chicago)

  Chicago 2004 - 2008 Bachelors

Réseau

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