CEA Saclay
- Chercheure en Microbiologie et Biologie moléculaire
Gif-sur-Yvette
2006 - maintenant
Expression hétérologue des hydrogénases à [Fe-Fe] et caractérisation enzymatique de la production d'hydrogène moléculaire.
PUBLICATIONS
1) Sybirna K, Bottin H. (2013) New insights into the systems for heterologous synthesis and maturation of the hydrogenases, the most efficient biohydrogen producers. Int. Journal of Hydrogen Energy. v. 38, p. 13164–13171.
2) Fourmond V, Baffert C, Sybirna K, Lautier T, Soucaille P, Meynial-Salles I, Bottin H, Léger C. (2013) Steady-state catalytic wave-shapes for 2-electron reversible electrocatalysts and enzymes. J Am Chem Soc. v. 135, p. 3926−3938.
3) Sybirna K, Ezanno P, Baffert C, Léger C, Bottin H. (2013) Arginine171 of Chlamydomonas reinhardtii [Fe-Fe] hydrogenase HydA1 plays a crucial role in the electron transfer to its catalytic center. Int. Journal of Hydrogen Energy. v. 38, p. 2998-3002.
4) Fourmond V, Baffert C, Sybirna K, Dementin S, Abou-Hamdan A, Meynial-Salles I, Soucaille P, Bottin H, Léger C. (2013) The mechanism of inhibition by H2 of H2-production by hydrogenases. Chem. Comm. v. 49, p. 6840—6842.
5) Baffert C, Sybirna K, Ezanno P, Lautier T, Hajj V, Meynial-Salles I, Soucaille P, Bottin H, Léger C. (2012) Covalent Attachment of FeFe Hydrogenases to Carbon Electrodes for Direct Electron Transfer. Anal Chem. v. 84, p. 7999-8005.
6) Shi L, Belchik SM, Plymale AE, Heald S, Dohnalkova AC, Sybirna K, Bottin H, Squier TC, Zachara JM, Fredrickson JK. (2011) Purification and characterization of the [NiFe]-hydrogenase of Shewanella oneidensis MR-1. Appl Environ Microbiol. v. 15, p. 5584-90.
7) Baffert C., Bertini L., Lautier T., Greco C., Sybirna K., Ezanno P., Etienne E., Soucaille P., Bertrand P., Bottin H., Meynial-Salles I., De Gioia L., Léger C. (2011) CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study. J Am Chem Soc. v. 133, p. 2096-2099.
8) Sybirna K., Antoine T., Lindberg P., Fourmond V., Rousset M., Mejean V., Bottin H. (2008) Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii. BMC Biotechnology, 8:73.
Université Paris-Sud
- Doctorante en Génétique Cellulaire et Moléculaire
Orsay
2002 - 2006
Evolution structurelle et fonctionnelle du gène HAP4; régulateur central de l’équilibre
fermentation/respiration chez la levure Saccharomyces cerevisiae.
PUBLICATIONS
1) Sybirna K., Petryk N, Zhou YF, Sibirny A and Bolotin-Fukuhara M. (2010) A novel Hansenula polymorpha transcriptional factor HpHAP4-B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif. Yeast. v. 27: p. 941–954.
2) Sybirna K., Guiard B., Li YF., Bao WG., Bolotin-Fukuhara M., Delahodde A. (2005) A new H. polymorpha HAP4 homologue which contains only the N-terminal conserved domain of the protein is fully functional in S. cerevisiae. Current Genetics, v. 47, p.172-181.
Institut de Biologie Cellulaire, Académie Nationale des Sciences d'Ukraine, Lviv, Ukraine
- Ingénieur de recherche en Génétique Moléculaire
2000 - 2002
Clonage des gènes de structure qui participent à la biosynthèse de la riboflavine chez la levure Candida famata.
PUBLICATIONS
1) Dmytruk K., Abbas C., Voronovsky A., Kshanovska B., Sybirna K., Sibirny A. (2004) Cloning of structural genes involved in riboflavin synthesis of the yeast Candida famata. Ukrainian Biochemical Journal, v.76, p.78 - 87.
2) Voronovsky A., Abbas C., Dmytruk K., Ishchuk O., Kshanovska B., Sybirna K., Gaillardin C., Sibirny A. (2004) Candida famata (Debaryomyces hansenii) DNA sequences containing genes involved in riboflavin synthesis. Yeast, v. 21, p.1307-1316.
3) Voronovsky A., Abbas C., Fayura L., Kshanovska B., Dmytruk K., Sybirna K., Sibirny A. (2002) Development of a transformation system for the flavinogenic yeast Candida famata. FEMS Yeast Research, v.2, p. 381-388.
BREVETS
1) Abbas C, Voronovsky A, Fayura L, Kshanovska B, Dmytruk K, Sybirna K, Sibirny A. (2006) Transformation system for flavinogenous yeasts. US Patent N°: 7009045.
