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Léa ANGUENOT

LAUSANNE

En résumé

Recently graduated student from EPFL, I am looking for a position in the sectors of medical devices, biotechnologies or pharma.
I would like to show and train my skills of fast learning, multi-tasking and troubleshooting in a vibrant environment. Used to integrate new groups, I am eager to take part to a multidisciplinary team in which I can also work in an autonomous way.

Mes compétences :
Culture cellulaire
C++
Immunostaining
Techniques de laboratoire
Biotechnologies
Immunophenotyping
Cancer diagnosis
LaTeX
ImageJ
SolidWorks
MATLAB

Entreprises

  • Lunaphore technologies SA - R&D assistant

    2016 - 2017 Lunaphore Technologies is a young start-up company active in the in vitro diagnostics field developing a product based on a microfluidic technology to analyse tumor samples. In the frame of Lunaphore's activities in the R&D department, there is a need to develop, optimize and test new protocols.

    I had to develop and implement startup’s projects, important for the future development of the company. My role was on one hand to improve existing protocols with the platform and to test detection kits in order to make important collaboration with partners. On the other hand, I was in charge of developing new protocols with the platform to show proof-of-concept results. This includes, scientific research, protocol execution, image acquisition, image processing, results analysis and reporting to the managers. In addition, I participated in giving important feedbacks and contribute in the continuous improvement of the instrument.

    Scope of work / Tasks
    • Test and develop new protocols of multiplexing
    • Execute and improve existing protocols/kits of immunostaining
    • Test and clean the microfluidic device prototypes
    • Improve and troubleshoot the microfluidic device prototypes
    • Tissue slide imaging with fluorescence or bright-field microscopes
    • Image processing with ImageJ
    • Result analysis
    • Collaboration and reporting to the lab team with oral and written
    presentations
    • Contribution to the quality system of the company

  • Rheon medical SA - R&D assistant

    2016 - 2016 Rheon Medical is a Swiss company founded in 2010 and based in the EPFL campus in Lausanne, Switzerland. Rheon focuses on the development of first-in-class glaucoma drainage devices, providing precise intraocular pressure control over the life of the device. Our goal is to deliver optimal solutions to surgeons, eliminating complications and treatment failures.

    I was in charge of the development of a new seton tube design for the glaucoma drainage device based on the issues observed on the first patients already having an eyeWatchTM. Then, I characterized the cell adhesion and proliferation on different coatings to investigate wound healing after the surgery.

    Scope of work / Tasks
    • Cell culture (mouse embryo fibroblast) to test different hydrophilic coatings
    • Literature research and brain storming about wound healing
    • Design and machining of mould using softwares
    • Polymer moulding in clean room
    • Cell result analysis
    • Collaboration and presentation of research results to the team
    • Visualization of the operation on a patient

  • Laboratoire d'Hémodynamique et Technologies Cardiovasculaires (LHTC) - Project intern

    2015 - 2015 Erectile dysfunction (ED) is a common, multi-causal disorder whose most prevalent risk factor is hypercholesterolemia. This situation leads to the development of atherosclerosis and rises various functional and structural alterations in the vasculature. Apelin was found to play a critical role in erectile function as its trigger the release of a vasodilator neurotransmitter.

    My project was to analyze the actions of long-term treatment with Apelin-13 in hypercholesterolemic-induced ED mice by evaluating key markers for erectile function. The result of this extensive study were significant and lead to a publication in 2016 in the Journal of Sexual Medicine.

    Scope of work / Tasks
    • Lab work: Western Blot and immunostaining
    • Penis tissues and blood samples analysis
    • Images acquisition with the bright field microscope
    • Analyzing and interpreting of research data and comparison with current
    literature
    • Collaboration and reporting to the lab team
    • Organization of the writing of the publication

Formations

  • Ecole Polytechnique Fédérale De Lausanne (EPFL) (Lausanne)

    Lausanne 2015 - 2017 Master

    • Bio-mécanique du système cardio-vasculaire et musculo-squelettal
    • Bio-miscroscopie, Sciences des matériaux
    • Analyse de la locomotion, computational motor control (Matlab), méthodes numériques en biomécanique (COMSOL)
    • Projet d'ingénieur en entreprise

  • Ecole Polytechnique Fédérale De Lausanne (EPFL) (Lausanne)

    Lausanne 2012 - 2015 Bachelor

    • Analyse, Physique, Algèbre, Probabilités et Statistiques
    • Programmation (C++), Modélisation (Matlab)
    • Electrique et électronique, Mécanique des fluides, Mécanique des structures
    • Biologie: Immunology, Neurosciences, Cancérologie, Physiologie, Biologie du développement, Biologie cellulaire et moléculaire, Stem cells

Réseau

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