Lucile HOCH


En résumé

Specialties : pharmacology, cell biology, biochemistry, molecular biology, drug discovery

Mes compétences :
Microsoft Word
Microsoft PowerPoint
Microsoft Outlook
Microsoft Excel


  • I-STEM - Research Associate

    CORBEIL ESSONNES 2015 - maintenant Limb-Girdle Muscular Dystrophy (LGMD) : Disease modeling and Drug Discovery

    - Cell Culture, Differentiation, Transfection, Nucleofection, Lentivirus Transduction
    - Induced Pluripotent Stem Cells
    - Immunofluorescence
    - Screening of small molecules libraries
    - High Content Microscopy (ImageXpress, CX7)
    - Bravo Liquid Handling Platform
    - Molecular Biology, Western Blot, ADN/ARN extraction, qPCR
  • CNRS - Doctorat, Ph.D

    Paris 2011 - 2015 Targeting the Hedgehog pathway in cancer
    Molecular and Pharmacological studies of the Smoothened receptor

    - Cell Biology
    - Immortalized cell lines, Primary culture, Transfection
    - Pharmacological studies
    - Drug discovery
    - Differentiation Assays (Alkaline phosphatase, Tecan microplate reader)
    - Bioluminescence Assays (Luciferase, Berthold microplate reader)
    - [3H]-Radioligand Binding Receptor Assays (Brandel)
    - Proliferation Assays ([3H]Thymidine incorporation)
    - GraphPad Prism IC50, EC50
    - Immunohistology, Fluorescence Microscopy
    - Molecular biology
    - Western Blot, Electrophoresis, ADN /ARN Extraction, Site-Directed Mutagenesis, PCR
    - Animal Testing, mice and rats (injection, perfusion, dissection)

    4 Publications
    3 Reviews

    3 years Grant from La Ligue contre le Cancer
    1 year Grant from La Fondation pour la Recherche Médicale
  • CNRS - Master 2 internship

    Paris 2010 - 2011 Development of Hedgehog signaling pathway inhibitors for the treatment of Hh-linked cancers
    Development of a radioligand to study the pharmacology of the Smoothened receptor

    - Cell Biology
    - Molecular Biology
    - Pharmacology
    - Alkaline Phosphatase and Luciferase Assays
    - Radioligand Binding Assay
  • INRA - Master 1 internship

    Paris 2010 - 2010 Study of two markers of nuclear remodelling during embryogenesis after different conditions of cloning

    - Cell Biology
    - Molecular Biology
    - Western Blot
    - Immunofluorescence