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Sanofi
- Research Associate
Paris
2017 - maintenant
Implement microfluidics platform for antibodies discovery. Construct antibody sequence libraries by droplet microfluidics technology coupled with NGS.
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University of Campinas, Brazil
- Teaching Assistant in Mass Transfer
2014 - 2014
Teaching Assistant in Mass Transfer at the School of Chemical Engineering, University of Campinas, Brazil. Taught classes on the topic of mass transfer, made and corrected exams with Professor, taught support classes.
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University of Campinas, Brazil
- Teaching Assistant in bioengineering
2013 - 2013
Teaching Assistant in bioengineering at the School of Chemical Engineering, University of Campinas, Brazil. Taught some topics of bioengineering, made and corrected exams with Professor, taught support classes.
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University Center of Educational Foundation of Barretos, São Paulo, Brazil
- Substitute Professor
2013 - 2013
Substitute Professor at the School of Chemical Engineering, University Center of Educational Foundation of Barretos, Brazil. Taught theory and practical classes of bioengineering.
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Ecole polytechnique
- PhD student
91128 PALAISEAU Cedex
2013 - 2017
This project aims to develop droplet-based microfluidic systems to incorporate nucleic acids into cationic liposomes and to transfect mammalian cells in vitro. The first step of the project took place in Brazil and involved the development of a microfluidic droplet system that promotes rapid mixing in order to incorporate pDNA into cationic liposomes, generating reproducible and suitable complexes for biological applications, like gene therapy. The second step of the project took place in France and involved the development of a microchip to transfect mammalian cells in vitro within soft 3D droplets of hydrogel, using the complexes as nanovectors. With this system, it is possible to investigate key parameters that influence the process of transfection in mammalian cells, which could be a potential tool for therapeutic vaccines.
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University of Campinas, Brazil
- Master student
2010 - 2013
This research aimed at the development of a production process for cationic liposomes for use in cancer immunotherapy strategies. Dendritic cells (DCs) vaccine has the function of helping the immune system to identify tumor antigens in order to eliminate cancerous cells. Cationic liposomes, in addition to activated DCs, are able to safely mediate the introduction of nucleic acids into cells. Through this strategy, total RNA extracted from tumor cells were incorporated into cationic liposomes, which were introduced in vitro into DCs. As a result, we developed a scalable process to produce cationic liposomes with physico-chemical characteristics that lead to their internalization by DCs, stimulating and delivering nucleic acids to cells.
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Clos Sainte Apolline - Domaine du Bollenberg, France
- Trainee at a beverage company
2009 - 2010
Participated in wine and "eaux-de-vie" process production, following their quality by check point analysis from harvest until the final product, passing by fermentation and distillation processes. Commercialization of the products in wine shops in France and Switzerland.
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Frigorífico Industrial Vale do Piranga, Oratórios, MG, Brazil
- Trainee at meat industry
2009 - 2009
Developed activities in the quality control sector of the pork industry in order to review GMP procedures and implement the HACCP plan.
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Alimentos Júnior, Viçosa, MG, Brazil
- Consulting services to meat industry
2008 - 2008
Rendered consulting services to the meat industry in order to develop a sausage nutrition label and standardize their production following GMP procedures.
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Indústria Favo de Mel, Belo Horizonte, MG, Brazil
- Trainee at ice cream industry
2008 - 2008
Developed activities in the quality control sector of the ice cream industry in order to review GMP procedures.
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Federal University of Viçosa, Brazil
- Internship at food microbiology laboratory
2007 - 2009
Isolated Escherichia coli O157:H7 from milk and cattle farms and evaluated antibacterial activity of sanitizers against biofilms on surfaces in the food industry. Investigated antibacterial effect of natural extracts and essential oils against campylobacter strains. Isolated Salmonella enteritidis bacteriophages from poultry farms in order to be used as biocontrol in meat industry.
