Mes compétences :
Brain Tumours
genetics
bioinformatics experience
Western Blotting
trasncriptome
Molecular genetics
teaching
Cell Culture
Entreprises
Yale University (USA)
- Associate research scientist
2010 - maintenantPrincipal projects:
-Investigation of the genomic and molecular components of brain tumors (Vestibular schwannoma)
-genetic of intracranial aneurysm.
• Designed, analysed and interpretated Exome Sequencing and expression micro arrays data.
• Established a stable cell line as a model to study brain tumor (Implemented under University rules and regulations)
• Implemented a quantitative-PCR method for validation of DNA copy number variation. Method adopted as routine screening by the team.
• Established a Drosophila model of brain tumor by learning RNAi knockdown system in Drosophila. Exposed to CRISPR/Cas9 technology in zebrafish.
• Collaborated with Istanbul University Cerrahpaşa Medical School for genetic causes of skeletal dysplasia with Pr. Beyhan Tüysüz.
• Consulted with the Endocrinology and Bone Center at Yale University for the identification of the gene implicated in a child showing an over proliferation of bone mass. Results led to a Research Grant (R21) awarded to Pr. Karl L. Insogna.
• Intensive experience in teaching and mentoring more than 20 students.
King's College London (UK)
- Research associate
2007 - 2010Principal project : Gene expression of brain aneurysms.
Part of the biological work package team in the @neurIST consortium ( 28 interdisciplinary members across Europe to provide an integrated decision support system to assess the risk of
aneurysm rupture in patients and to optimize their treatments www.aneurist.org/).
* Designed and performed the gene arrays experiments and analyzed High-throughput gene expression
experiments. Worked with a computational scientist to design the pipeline for data analyses.
* Supported budget and financial monitoring. ;
* Wrote reports and deliverables for the consortium. ;
* Collaborated with the team in Paris (genetic), Geneva (clinical), Barcelona (consortium meetings),
Freiburg (computational biology), and Sheffield (clinical).
* Organized human clinical samples following UK Human Tissue Act regulations, coordinated with EU
clinical centers for sample and clinical information retrieval.
* Co-organized tutorials for PhD and postdoctoral fellows in the vascular biology department (Grant
awarded by KCL: GBP 5000)
* Collaborated with the charitable company, St. Stephen's AIDS Trust. Investigated the effect of lowdose Ritonavir on healthy volunteers. Designed the project and analyzed pharmacogenomics data.
Centre Hospitalier Universitaire de Nancy
- PhD student
2003 - 2007Principal Project: Identification of gene candidates in an orphan disease (Aicardi Syndrome).
• Designed, performed the experiments, analyzed the data (CGH array and Agilent technology two color gene expression array). Locations: Human Genetics Laboratory (France) and The Thrombosis Research Institute (UK).
• Lectured forFrench diploma for laboratory technician in human genetics (DNTS). Courses title: Biological Databases and their application in human genetics. Location: Institute of Technology of Brabois (Nancy, France)
• Worked on the implementation of ACGR (Aicardi Candidate Gene retrieval), a software designed to find candidate gene from biological public databases. Designed the project, followed and tested the implemented software. Location: LORIA (Laboratoire lorrain de recherche en informatique et ses applications). INRIA (French national institute for research in computer science)(France).
