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Institut de Génétique Humaine / CNRS
- Research Assistant
MONTPELLIER
2015 - maintenant
Molecular Basis of Cancer Related Inflammation
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Inserm
- Ingenieur d'étude
PARIS 13
2011 - 2014
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Centre de biochimie structurale, INSERM
- Ingenieur D'etude
2010 - 2014
DNA segregation and remodelling group
Architecture and Assembly of chromatin in eukariotic cell by super resolution microscopy
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Massachusetts General Hospital, Harvard medical school
- Lab manager
2007 - 2009
Dept Innate immunology, Laboratory of Dr L Stuart.
Project: rac2, regulator of innate immune signaling that can tailor the immune response to bacterial virulence determinants.
Techniques used: QRT PCR, cell culture, transfection, luciferase assay, RNAi, drosophila work, GATEWAY cloning
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Joslin Diabetes Center Affiliate at SETMA
- Research assistant/ lab manager
2005 - 2007
Research assistant, Lab Manager,
Dept Molecular and cellular biology, Laboratory of Dr RN Kulkarni,
Assay core: ELISA, RIA, cell culture, binding assay. insulin, C-peptide, leptin, glucagon, ICAM, VCAM, TNF-alpha, C-reactive protein, Interferon-gamma, Interleukins -2, -4, -5 and -10.
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CNRS
- Technician
Paris
1999 - 2002
Research assistant, Laboratory of Dr. J Martinez, CAPS Project, Computer Assisted Peptide Search.
Involved in the biological part of this project.
Commonly used techniques:
- Bacterial culture, transformation, DNA extraction, PCR, Immunoblotting.
- Mammalian cell culture, transfections (electroporation, lipofection, calcium phosphate methods).
- Cell Proliferation Analysis by [3H]Thymidine Incorporation.
- Gene expression dosage (luciferase assays) (work experience on various of assays in 96-well format).
- Binding and competition assays: Radio Immunosorbent Assays, cell based second messenger assays.
- Cell/tissue membranes purification.
- Radioactive labelling in situ on cerebellum sections.
