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Arnaud GIESE

BORDEAUX

En résumé

Compétences techniques
Biochemical and analytic analysis Biochemistry
Molecular and Cellular biology
Epifluorescence/Confocal/STED Microscopy
Mouse and Rats inner ear microdissection, cochlea in vitro electroporation, Cellular culture, Primary culture
Cryosection
Statistical analysis of experimental data

Entreprises

  • Cincinnati Children's Hospital Medical Center - Post-doctorat chez Division of Otolaryngology Head & Neck Surgery

    2011 - maintenant

  • INSERM U862, Institut François Magendie - Doctorant

    2007 - 2010 Regulation of the core planar cell polarity protein Vangl2 in the organ of Corti

    Several epithelia exhibit a second polarity perpendicular to the apico-basal axis, called planar polarity and that governs the orientation of structures such as stereocilia and hear. Our laboratory studies planar polarity, using mammalian cochlear sensory epithelium and we focus our studies on Vangl2, that we identified as the first mammalian planar polarity gene. Vangl2 encodes a four-transmembrane protein that contains a PDZ binding domain in its C-terminus tail. Vangl2 is asymmetrically located at the junction between mechanosensory hair cells and supporting cells, and this asymmetry appears important for planar cell polarity. I have shown in my thesis, using STED microscopy, that Vangl2 asymmetry is mainly due to an accumulation of Vangl2 to the distal side of supporting cells. I sought to dissect the molecular role of Vangl2 by analysing its trafficking within the cochlear epithelium. Deletion analysis shows that the last 12 amino acids, unlike its N-terminus tail are essential for Vangl2 endoplasmic reticulum sorting, its plasma membrane targeting and its function. Conditional mutant mice analysis show that Scrib1, which we have previously shown, interacts with Vangl2 through the PDZ binding domain of its C-terminal tail, is not the protein mediating this asymmetry. My work also highlight that GIPC1 had a role in the regulation of PCP and maintaining the integrity of hair bundles of sensory cells, and that the complex GIPC1/Myosin VI could regulate Vangl2 asymmetry in the organ of Corti.

  • Composante Innée de la Réponse Immunitaire et Différenciation, CNRS UMR 5164 - Etudiant

    2006 - 2007 Master 2

  • Adaptation cardiovasculaire à l’ischémie, INSERM U441, Hôpital Haut Lévèque - Etudiant

    2005 - 2006 Master 1

Formations

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