Ecophysiologist of plant response to abiotic stress
specialized in elicitation, analysis and promotion of bioactive molecules in plant.
Biostumilant in sustainable agriculture
Mes compétences :
Agronomie
Biotechnologies
Biologie
Entreprises
Solvay
- Reaserch scientist
Paris2013 - 2014• Experiment design and conception of innovative ways to implement the new agro-polymers technology and to study their effects on germination, Plant, root development and plants symbiotic systems.
• Responsible for the ecophysiological and agronomical study of Solvay’s polymers effects on maize
- Quantitative Assessment of Phenotypic and physiological Plasticity
- Activity reports, protocols optimization, shelf life statements.
Université de montpellier 2
- Research associate
2012 - 2013• Designing and planning the research project.
• Extraction and analysing of plant secondary metabolites using HPLC, LC-MS/MS (ES+/-) and LC-LSD.
• Application of different chemicals, NO donors/H2O2 and their effects on mutants phenotype
• Ecophysiological measurements (Gas exchange, Flavonoids, chloroplast, total anti-oxidant index).
• Gene expression profiling of phenolic compounds pathway using qPCR
University of ryukyus
- Research scientist
2007 - 2008• Selection and culture of microalgae, epifluorescence microscopy, GC-MS,
• Experiments with Nitric oxide donors, APOLLO 4000, and different abiotic stress
• Proteins DNA sequence analysis (comparative genomics/proteomics, phylogeny)
• Heterologous protein expression in S.cerevisiae, protein extraction and analysis (SDS, 2DPAGE).
Cirad
- Molecular biologist-virologist
Paris2007 - 2007• Optimising and Conducting island-wide sampling of banana leaves.
• Banana leave DNA and RNA extraction, conducting molecular analysis for viral sequence detection using M-IC-PCR, IC-PCR, IC-DOP-RT/DB-DOP-RT NESTED PCR
Vandoeuvre Les Nancy2008 - 2011biologie végétale et forestière
• Planning and coordinating phytotronic ozone fumigation.
• Gas exchange, total chlorophyll measurements, biomass measurements, primary metabolite.
• Analysis of stress responses gene (PDF1, PR1, JAS1) (qPCR).
• Enzymatic characterisation and measurements of CO2 fixating and NAD(P)H dependent enzymes.
• Proteins DNA sequence analysis (comparative genomics, phylogeny)
• Heterologous protein expression, Protein sequencing and analysis SDS-PAGE, 2D PAGE/Measurement of NOS activity, NO detection using Fluorescence probes (Plant and microoganisms).