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ExplorAqua & Aluna Beach Lounge, Alona, Philippines
- Chief Executive Officer
2014 - 2015
ACTIVITY
Project manager
Management of 15 employees (waitresses; bartender, staff, security, handyman...)
Planning and team management
Direct interaction with supplier and customers
AIM
Creation, development and manager of two companies: Explor’Aqua (clear kayak rental) & Aluna Beach Lounge Bar
COMPETENCES
Management, marketing, advertising, sale, communication...
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PrAna Divers, Alona, Philippines
- Scuba Diving Instructor & Emergency First Response Instructor
2014 - 2015
• Teaching Human Physiology and Physic linked to scuba diving: circulatory & respiratory systems, decompression sickness, barotrauma, hyperbaric conditions, gas narcosis, oxygen toxicity...
• Teaching Emergency First Response: Primary and Secondary Care, Care for Children, cardiopulmonary resuscitation, automated external defibrillators...
• Teaching practical courses from Discover Scuba Diving up to Divemaster, Scuba Reviews, guiding
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PrAna Divers
- Scientific Officer
2014 - 2015
ACTIVITY
Project Manager
Responsible for Scien:fic Technical Support
Manage 7 collaborators
Organize the activity and planning teams
Improve the activity of the team by developing strategies and tools to be more efficient
AIM
Creation & development of an artificial coral reef
COMPETENCES
Marine biology, management, marketing, advertising, communication...
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Liver Disease and Carcinogenesis Laboratory, University of Western Australia, Australia
- Research Associate
2013 - 2014
ACTIVITY
Research projet manager on hepatocarcinogenesis process: Study BARD1 isoform involvement in transformation of Liver Progenitor Cells (LPCs) into tumorigenic LPCs.
Management of 10 collaborators (From technician to PhD levels)
Planning and team management
Direct interaction with technical supplier, MD...
AIM
My main research area was the understanding of the hepatocarcinogenesis process. We hypothesis that BARD1 isoform expression is responsible for marked chromosomal alterations observed in tumorigenic Liver Progenitor Cells (LPCs) and this directly drives the tumorigenic process. The aims of this project is: to determine BARD1 isoforms expression profiles in tumorigenic and non-tumorigenic LPCs; to demonstrate that forced over-expression or repression of BARD1 isoforms that are highly expressed or suppressed in tumorigenic LPCs will transform non-tumorigenic LPCs; and to demonstrate similar alterations in BARD1 isoforms occur in vivo. This experience in this team allowed me to improve my knowledge in carcinogenis, so in the cell cycle regulation, and also in the stem cells domain.
COMPETENCES
plasmid construction, cloning, subcloning, stable/transient transfection by Lipofectamine, FuGene, Effectene, Reverse Transcription, PCR (Clean-up, Inverse, Nested, quantitative), DNA and RNA isolation, Southern and Northern Blot, protein extraction and purification, gel electrophoresis, concentration determination of nucleic acids and proteins using spectrophotometer (Bradford & Lowry assays...), Western blotting, immunocytochemistry, immunohistochemistry, immunofluorescent double/triple staining, flow cytometry, cell culture...
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Unit of Research on Cornea and Lens, Jules-Gonin Eye Hospital, University of Lausanne, Switzerland
- Postdoctoral Research Fellow
2010 - 2012
ACTIVITY
Research projet manager: Study effects of ROCK inhibitor Y-27632 on proliferation/migration/adhesion and wound healing ex vivo and in vitro of human corneal endothelial cells (HCEC)
Management of 5 collaborators (From technician to PhD levels)
Planning and team management
AIM
My main ophthalmic research area has so far been the study of the improvement and the survival of corneal transplants in organ culture. First of all, my aim was to evaluate the action of Rho-associated serine/threonine kinases (ROCKs), major effectors of Rho GTPase, in these cells in vitro and ex vivo. My research team and I demonstrated that ROCK activities enhance cell proliferation, negatively modulate cell migration and cell adhesion and therefore play a role in regulating corneal endothelial wound healing.
Then, I worked on:
• hTERT overexpression by lentiviral vector transduction on HCEC
• the role of C/EBPd on the HCEC cell cycle regulation
• the role of the environment on the cell fate of corneal epithelial stem cell
COMPETENCES
plasmid/lentiviral plasmid construction, cloning, subcloning, stable/transient transfection by Lipofectamine, FuGene, Effectene, lentivirus, siRNA, antisense, Reverse Transcription, PCR (Nested, quantitative), DNA and RNA isolation, protein extraction and purification, gel electrophoresis, concentration determination of nucleic acids and proteins using spectrophotometer, primary HCEC isolation, cell lines/primary cell culture (human, mouse, pig, rabbit), cell growth curve, cell adhesion and migration assays, wound healing, cell apoptosis and differentiation assays, cytotoxicity and cell viability assays, proliferation assays, cell sorting by FACS, senescence assays, cell metabolism determination, Western blotting, immunocytochemistry, immunohistochemistry, immunofluorescent double/triple staining, flow cytometry, lentiviral gene transfer (plasmid construction, virus production, purification, titration & transduction)...
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Institut de Formation en Soins Infirmiers (IFSI ; Red Cross) School of Nursing, Saint Etienne, Franc
- Lecturer
2009 - 2011
Teaching Immunology & Infectiology
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Clinical Research Division, Ophthalmology Dpt., University Hospital, Saint Etienne, France
- Clinical Research Associate
2009 - 2011
ACTIVITY
Supervision of two clinical trials
o Comparative study of two preservation medium for corneal transplants: reference media vs.
media without animal compounds
Prospective Multicentric Randomized Clinical Study
o Curarization effects on the oculomotricity in non strabismic children Therapeutic Comparative Randomized Multicentric study
Monitoring of different clinical trials
o GEFAL Study: Groupe d’Evaluation Français Avastin® versus Lucentis®
o Interest of the corticosteroid therapy in first intention, by intravitreal injections during post- operative endophtalmitis
o Contribution of the fast molecular bacterial identification by real time PCR in the care of the
acute post-operative endophtalmitis
o LT2345- PIII-12/08 Study: Efficacy and safety assessment of t2345 unpreserved eye drops
versus Xalatan® in ocular hypertensive or glaucomatous patients
o Protocole 192371-016-00: Allergan Cyclosporine & AKC
AIM
Once my Clinical Research Associate Diploma obtained during my PhD, I worked as main CRA in various Ophthalmological Clinical trials which allowed me to acquire a solid experience in clinical research and to deepen my competences in translational research.
COMPETENCES
Writing and review protocols, CRF’s, data management plans, study reports, and informed consents developing and writing trial protocols according to ICH/GCP guidelines; Presentation trial protocols to a steering committee; Design data collection forms (CRFs); Coordination with the ethics committee; Liaison with doctors/consultants (or investigators) on conducting the trial; Monitoring the trial throughout its duration and visit of the study centers; Review regulatory documents; Collect completed CRFs from hospitals and general practices; Writing visit reports; Fill and collate trial documentation and reports; Close down study centers on completion of the trial)
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Institut de Formation en Soins Infirmiers (IFSI ; Red Cross) School of Nursing, Saint Etienne, Franc
- In charge of Academic formation
2009 - 2010
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‘Biology, Engineering and Imaging of corneal graft’ Laboratory, Faculty of Medicine, Saint-Etienne,
- Doctoral student in Molecular & Cellular Biology
2006 - 2010
ACTIVITY
Research projet manager: Study of the human corneal endothelial cells (HCEC) cell cycle regulation
Management of 5 collaborators (From technician to PhD levels)
Planning and team management
AIM
During my PhD. preparation I also contributed to other Ophthalmic projects, such as the study of the cell cycle regulation of human corneal endothelial cells and the improvement of corneas survival conserved in organ culture. For this, I developed several techniques which were not used in the laboratory before my arrival, an innovative device that allows gene electrotransfer. Furthermore, in order to evaluate the endothelial cell viability on predissected endothelium grafts by an eye bank and sent to a remote location I developed an original method for pan-corneal endothelial viability and toxicology assessment. I parallel to these studies, I looked for new compounds for deswelling organ culture corneas and new vital dyes to improve the quality control of the corneal endothelium of stored corneas
COMPETENCES
Primary HCEC isolation, cell lines/primary cell culture (human, mouse, pig, rabbit), cell apoptosis and differentiation assays, cytotoxicity and cell viability assays, proliferation assays, cell sorting by FACS, senescence assays, plasmid construction, cloning, subcloning, stable/transient transfection by Lipofectamine, FuGene, Effectene, lentivirus, Nucleofector and Electroporation, siRNA, antisense, Reverse Transcription, (q)PCR, DNA and RNA isolation, MicroArray, protein extraction and purification, gel electrophoresis, concentration determination of nucleic acids and proteins using spectrophotometer (Bradford & Lowry assays...), Western blotting, immunocytochemistry, immunohistochemistry, immunofluorescent double/triple staining, light microscopy, fluorescent microscopy, video microscopy, time- lapse microscopy, Multiple Images Acquisition (MIA), Extended Focus Images (EFI)...
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Laboratoire Arago, UPMC-Paris 6 and CNRS, FRE 3247 & GDR 2821, Banyuls s/ Mer, France
- Postgraduate Training Course (Master 2)
2006 - 2006
AIM
Study of the tissular expression and enzymatic characterization of arylalkylamine N- acetyltransferase (AANAT) in Scyliorhinus canicular
COMPETENCES
Luciferase assay, ELISA, pull down assays, immunoprecipitation, Western blotting, flow cytometry, protein activity assays, DNA recombination, plasmid construction, cloning, subcloning, Reverse Transcription, PCR (Clean-up, Inverse, Nested, quantitative), DNA and RNA isolation, protein extraction and purification, recombinant protein, gel electrophoresis, fusion protein, concentration determination of nucleic acids and proteins using spectrophotometer (Bradford & Lowry assays...)...
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Laboratoire Arago, UPMC-Paris 6 and CNRS, FRE 3247 & GDR 2821, Banyuls s/ Mer, France
- Postgraduate Training Course (Master 1)
2005 - 2005
AIM
Study of the aanat-2 gene expression and the starting of the circadian oscillator in Danio rerio larvas and embryos
COMPETENCES
in situ hybridization, DNA and RNA isolation, Southern and Northern Blot, gel electrophoresis, animal experimentation, microscopy, Luciferase assay, ELISA, Western blotting, immunohistochemistry...
