Clara LEVREL

En résumé

Entreprises

• Ministère De L'education Nationale - High School blind teacher’s assistant and middle school supervisor

  Paris 2017 - maintenant Levi-Strauss Middle School and Fenelon High School.
  
  Responsibilities :
  -student supervision and security
  -administrative gestion
  -pedagogic assistance for students and the teacher
  -class preparation and copy correction

• Inserm - Engineer assistant

  PARIS 13 2015 - 2016 Team " Dyslipidemia and lipotoxicity "
  
  The type 2 Berardinelli-Seip lipodystrophy is a rare disease affecting the Bscl2 gene coding for the seipin protein. BSCL2 disease have major consequences on the adipose tissue metabolism and integrity.
  
  My work consisted to investigate the consequences of the knock down of the Bscl2 gene expression in a new model of differentiated mature adipocytes. We researched for the cellular signalisation involved, protein partners of the seipin and the involvement of calcium and lipid homeostasis in the phenotype.
  
  Responsibilities :
  
  -Experiments development, conception,
  -Hypothesis definition and validation.
  -Data collection, analysis and interpretation
  -Bibliography, scientific watch
  -Intern and extern communication
  
  
  Achievement :
  
  -Characterization of a novel pathway involved in Bscl2 phenotype.
  -Development of a new protocol for live calcium imaging in 3T3-L1 differentiated adipocytes.1
  
  
  Mots clés : Conception, réalisation, expérience, hypothèse scientifiques, validation, collecte des résultats, analyse, interprétation, communication interne externe, veille scientifique, bibliographie .

• Inserm - Master degree Internship

  PARIS 13 2015 - 2015 Team : "Dyslipidemia and lipotoxicity »
  
  
  The type 2 Berardinelli-Seip lipodystrophy is a rare disease affecting the Bscl2 gene coding for the seipin protein. BSCL2 disease have major consequences on the adipose tissue metabolism and integrity.
  
  Characterization of the new cellular model of inducible knock down of the Bscl2 gene by shRNA in infected 3T3-L1. Validation of the model formed by cell phenotype, gene expression profile and cell viability. First search for signaling pathways involved (i.e. calcium).
  
  Responsibilities :
  
  -Experiments development,
  -Data collection, analysis and interpretation
  -Bibliography
  
  Achievement :
  
  - Validation of the model by phenotyping, gene expression and cell viability
  -2nd writer of « FGF21 Improves the Adipocyte Dysfunction Related to Seipin Deficiency » Dollet L and al, Diabetes, Nov 2016.
  -Master thesis

• Inserm - Master Internship

  PARIS 13 2014 - 2014 Team « Dyslipidemia and lipotoxicity »
  
  Formation of a mature adipocyte seipin KD cellular model.
  
  The type 2 Berardinelli-Seip lipodystrophy is a rare disease affecting the Bscl2 gene coding for the seipin protein. BSCL2 disease have major consequences on the adipose tissue metabolism and integrity.
  
  My work consisted in the formation of a new cellular model of adipocyte with an inducible knock down of the Bscl2 gene by lentiviral infection of 3T3L1 cells.
  
  Responsibilities :
  
  -Experiments development,
  -Data collection, analysis and interpretation
  -Bibliography
  
  Achievement :
  
  -Development of the cellular model
  -Bachelor thesis

Formations

• Université De Nantes - UFR Sciences Et Techniques

  Nantes 2014 - 2015 Master’s degree in BBTR (Biology, Biotechnology and Therapeutic Research)
  
  With high honors

• Université De Nantes - UFR Sciences Et Techniques

  Nantes 2013 - 2014 Master degree

• Université De Nantes - UFR Sciences Et Techniques

  Nantes 2010 - 2013 Bachelor in Biochemistry and Biology
  
  With honors

Réseau

• Katell LEON

• Marcel MARC