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Daniel PISSALOUX

LYON

En résumé

Medical oncology:
Technologies applied to diagnosis / prognosis / treatment of tumors: FISH, aCGH, PCR,
Pathologies: carcinomas (breast / lung), sarcomas, melanocytic tumors, lymphomas,
MiRNA expression profiling (microarray / TLDA).

Molecular biology:
Nucleic acid and protein methods, molecular cloning,
Primer design, quantitative and multiplex PCR / RT-PCR / PCR,
Probe design (custom FISH and DNA chips).

Bioinformatics / bioanalysis:
Operating systems and softwares: Windows, Unix, MacOS, Microsoft Office,
Business software: R, Spotfire, SRS package, SDS, Primer Express, MapMaker, DNAstar, Sequencher, Vector NTI, CellQuest Pro, Tribvn, Applied Imaging CytoVision, Agilent Genomic Workbench, Cytogenomics,
Programming language: Perl, BioPerl, S+ via the R software.

Other skills:
Cellular Biology (culture, viral vectors, flow cytometry),
Genetic markers and cartography,
Microbiology, protein engineering, process engineering, biochemistry, immunology.

Languages:
French: native language,
English: professional working proficiency (IRL level 3).

Mes compétences :
Oncology
Molecular Cytogenetics (FISH, aCGH)
Molecular Biology
Bioinformatic and bioanalysis

Entreprises

  • Centre anticancéreux Léon Bérard (CLB) - Biomedical engineer

    2008 - maintenant Permanent contract in the Biopathology Department.

    « In charge of the molecular cytogenetic platform for cancer diagnosis (FISH and aCGH) at the Centre Léon Bérard »
    Validation of FISH and aCGH experiments. In charge of the organisation and the development of both platforms. Implementation of new technologies and new biomarkers for the diagnosis, the prognosis and the treatment of cancer. Conception and management of molecular databases.
  • Centre anticancéreux Léon Bérard (CLB) - Researcher exchange (2 months), European Network of Excellence Conticanet

    2008 - 2008 Institut Josephine Nefkens, Erasmus Medical Center, Rotterdam (the Netherlands).
    Laboratory of Translational Pharmacology, under the direction of Dr E. Wiemer.

    « Comparison and validation of two platforms for miRNA profiling (Taqman® Low Density Arrays and Exiqon chips): application to FFPE samples ».
  • INSERM U590 (CLB, Lyon) - Biological engineer

    2006 - 2008 3 years contract in the team “Cytokines et Cancer”, under the direction of Dr L. Alberti.

    « Protein profiling in leiomyosarcomas: bioanalysis of antibody chips »,
    « Improving the diagnosis of liposarcomas by quantitative PCR »,
    « Development and validation of new diagnosis tests by quantitative PCR on FFPE samples: clear cell sarcoma and desmoplastic sarcoma ».
  • INRA - Expert engineer in bioanalysis

    Paris 2005 - 2005 8 months contract in the team “Embryogénèse Précoce”, RDP laboratory, UMR 5667
    (ENSL/CNRS/INRA/UCBL), under the direction of Dr P. Rogowsky.

    « Study of the development, the filling and the desiccation of cereals », European program Genoplante.
  • Biogemma - Biological Engineer internship

    2004 - 2004 7 months contract in the team “Génomique Blé et Maïs”, under the direction of Mr P. Lessard at Biogemma (Limagrain group).

    « Bioanalysis of expression data from DNA chips to study the transcriptome of developing maize grain », European program Zeastar.
  • Bonduelle - Technician internship

    Villeneuve-d'Ascq 2003 - 2003 2 months contract in the Quality Control department , under the direction of Mrs C. Malesys.

    « Quality controls in production ».

Formations

Réseau

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