Geoffrey DE OLIVEIRA

En résumé

Entreprises

• Lonza Biologics - Scientist

  2016 - maintenant Scientist within the Lonza's Applied Protein Services department, Light Path Discovery group.
  Granta Park, Cambridge (United Kingdom).

• GlaxoSmithKline - Industrial Placement Student

  Marly-le-Roi 2015 - 2016 Third year internship
  Project carried out from July 20th 2015 to February 19th 2016
  
  GlaxoSmithKline Medicines Research Centre
  Biopharm Molecular Discovery
  Antibody Selections and Antibody Technologies Groups
  Gunnels Wood Road, Stevenage, Hertfordshire, United Kingdom
  
  Rob Prince's Team.
  Project supervisor : Stephanie Hopley.
  
  Project : Development of a high-throughput antibody variable gene sequencing approach to study the antibody diversity of transgenic animals.
  
  Skills: RNA/DNA extraction and purification (automated and manual), 5’RACE (Rapid Amplification of cDNA ends), RT-PCR, SOE-PCR, cloning (TA cloning, In-Fusion), high-throughput transformation in E.coli, antibody engineering, transfection in HEK cells and expression of antibodies. Protein A-based purification (ÄKTA Xpress). Quality control testing: SDS-PAGE, endotoxin analysis.
  Analysis of sequencing data using Lasergene (SeqMan Pro, SeqBuild, MegAlign) and BioEdit software.

• Darty - Service pole employee

  BONDY 2014 - 2015 Part-Time job student
  
  Mission : storekeeper, service department

• CNRS - Intern

  Paris 2014 - 2014 Internship at CNRS (National Centre for Scientific Research) in the team "Mitochondrial organization and dynamics" led by Manuel Rojo.
  
  Project : Expression, purification and characterization of Mitofusins proteins in Pichia pastoris.
  
  Developed skills :
  - Yeast culture
  - Extraction and subcellular fractionation
  - Purification methods : Affinity chromatography IMAC (Ni-NTA Qiagen, to target specifically the recombinant Mitofusin 2 protein with a 6-his tag), size exclusion chromatography (HiLoad Superdex 200 PG and Superose 6 used on AKTA Purifier and GE Healthcare UNICORN Software)
  - SDS-PAGE and BN-PAGE (Coomasie and Western Blot)
  - Analysis of GTP binding with mantGTP
  - Indirect measures of GTPase activity
  - Dynamic Light Scattering (on DynaPro Nanostar Wyatt)
  - Circular dichroism

• E.LECLERC Champfleury - Employé Commercial

  2013 - 2013 Mise en rayon liquides et gestion des commandes

• E.LECLERC Pierry - Employé Commercial Drive

  2012 - 2013 Emploi étudiant à mi-temps. Réception des commandes, gestion des livraisons aux clients

• E.LECLERC Pierry - Employé Commercial Drive

  2012 - 2012 Emploi étudiant à mi-temps. Réception des commandes, gestion des livraisons aux clients

Formations

• ENSTBB (Ecole Nationale Supérieure De Technologie Des Biomolécules De Bordeaux)

  Bordeaux 2013 - 2016 Ingénieur en biotechnologies
  
  ENSTBB is a french engineering school specialized in biotechnology. Students are selected from university or preparatory class to get an engineering degree (equivalent MSc) in 3 years.
  Completed degree with honours in June 2016.
  
  http://enstbb.bordeaux-inp.fr/
  
  Activities:
  -President of ENSTBB Students Union (called "Eïcosa") (2014-2015) : skills : team management, cost management, events managem

• Université De Reims, Faculté Des Sciences Exactes

  Reims 2010 - 2013 Licence Sciences et Techniques : Biologie, Biochimie, Terre et Environnement
  
  Parcours Biologie Cellulaire et Physiologie

• Faculté De Médecine De Reims

  Reims 2008 - 2010 Première année en Premier Cycle des Etudes Médicales à la Faculté de Médecine de Reims (Université de Reims Champagne-Ardenne)

• Lycée Léon Bourgeois

  Epernay 2004 - 2008 Scientific Baccalaureate
  
  Baccalauréat Scientifique, spécialité SVT, mention Assez Bien et Européenne Espagnol

Réseau

• Alice LALY

• Aymeric BUNEL

• Carole PÉROT

• Fanny BLAUDEZ

• Fanny LEPETIT

• Marion DELIGNY

• Pauline PEUZIAT

• Sophie BOURGOIS

• Sophie VAUD

• Suzy CHABERT