Mes compétences :
Cell Culture
statistical analysis
metabolite analysis
Western Blotting analysis
Western Blotting
SDS
Oncology
Minitab
Microsoft Office
Microsoft Excel
Mass Spectrometry
ELISA
DNA extraction
Biotechnology
Antibody
Entreprises
Novimmune SA
- USP Intern
2014 - 2015Project: Generation and selection of highly producing stable recombinant cell lines for the production of bispecific antibodies
Leading of a dedicated project
Autonomous plannification and performance of experiments
Team discussion about results and improvements of the process
* Mammalian cell culture (CHO-K1) and transfection, CHO cell line screening, CHO cell
subcloning, generation of clones in semi-solid medium using the ClonePix FL device
* Small scale fed-batch and batch fermentation ;
* Cellular biology: viability, metabolite analysis using the CuBiAn HT 270 ;
* Purification of mAb and quantification assays using the ForteBio's Octet®
Inserm unit U1035, Biotherapies of genetic diseases and cancer
- Intern
2013 - 2013Project: Development and optimization of the production and purification process of a recombinant protein
• Mammalian cell culture (HEK-293T), recombinant protein expression, SDS-PAGE and Western Blotting analysis, interpretation of mass spectrometry data, optimization of a batch purification process
ENSTBB
- Etudiant en fin d'étude et en recherche d'emploi