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Sanofi Pasteur
- PROFESSIONAL QUALITY CONTROL - ANALYTICAL EXCELLENCE
Lyon
2018 - maintenant
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QIMR BERGHOFER, HERSTON, QUEENSLAND (AUSTRALIA)
- RESEARCH ASSISTANT – G. AND J. GILMOUR LEUKAEMIA RESEARCH
2013 - 2016
Responsibilities:
Conduct experiments and analyze results, ordering, management of the mice strains.
Achievements:
- Setting up the CRISPR/Cas9 genome editing technique to study the role of oncogenes in the transformation of chronic myeloproliferative neoplasm to acute myeloid leukaemia.
- Training of new staff members.
- Improvement of the mouse database.
Technical skills:
DNA extraction, PCR, cell culture, cell staining and flow cytometry, blood smears and slide staining, cloning, virus production, purification, concentration and transfection, animal handling (IV, IP, SC and OG injections, retro-orbital bleeding, bone marrow transplantation, evaluation of health status, ear tagging/punching).
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GALDERMA R&D, BIOT, ALPES-MARITIMES
- JUNIOR RESEARCHER – ADVANCED PHARMACOLOGY
Courbevoie
2012 - 2012
Responsibilities:
Plan, design, conduct experiments and analyze results, ordering, search of scientific literature, write laboratory reports and present work at regular laboratory meeting.
Achievements:
- Improvement of an in vivo model for evaluation of dermal injectable products.
- Development of a new technique to follow inflammation by optical imaging.
- Writing a report about in vivo imaging technologies.
- Training of colleagues to use the IVIS Lumina II technology.
Technical skills:
RNA extraction, RNA quality control, RT-qPCR, cytokine dosage, in vivo bioluminescent and fluorescent imaging, histological analysis, immunohistochemistry, animal handling (IV, ID injections, oditest, tattooing).
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SANOFI PASTEUR, MARCY L’ETOILE, RHONE
- TECHNICAL COORDINATOR OF IMMUNOLOGY‐MICROBIOLOGY ‐ DISCOVERY
2011 - 2011
Responsibilities:
Plan, design, conduct experiments and analyze results, ordering, search of scientific literature, write laboratory reports and present work at regular laboratory meeting.
Achievements:
- Development and implementation of an in vivo model of enterobacterial infection.
- Development of technics to generate, characterize and visualize bioluminescent and fluorescent bacteria.
- Writing a report about in vivo models of an enterobacterial infection.
Technical skills:
Bacterial culture, staining and flow cytometry, colony blot, southern blot, cloning, heat shock transformation and electroporation, histology (fixing, embedding, sectioning, staining), animal handling (IV, IM, IP, OG, IR injections, submandibular bleeding).
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CHARLES RIVER, ST GERMAIN SUR L’ARBRESLE
- TECHNICIEN OF AUTOPSY/PARASITOLOGY – RESEARCH ANIMAL DIAGNOSTIC SERVICES
SAINT-GERMAIN-SUR-L'ARBRES
2010 - 2010
Responsibilities:
- Ensure technical realization, with respect of the GLP and deadlines. Achievement:
- Evaluation of the health status of animals from internal productions and
external customers.
Technical skills:
Autopsy and collection of biological samples for blood and bacterial analysis, research and identification of intestinal parasites and ectoparasites by microscopy, cardiac puncture.
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INSERM, LYON, RHONE
- RESEARCH ASSISTANT – IMMUNITY AND VIRAL INFECTION
2007 - 2008
Responsibilities:
Conduct experiments and analyse results, management of the mouse strains.
Achievements:
- Implementation of a Cre transgenic mouse line in order to study the role of TGF
beta in the regulation of the T-cells.
- Creation of a mouse database.
Technical skills:
PCR, autopsy and biological samples collection, cell staining and flow cytometry, cell culture, animal handling (anesthesia, euthanasia, toe clipping).
