Mes compétences :
Virologie
Data analysis
Transfert de technologies
Scale up
Biotechnologies
Bioréacteurs contrôlés
Cell culture
Expérimentation animale
Illkirch Graffenstaden Cedex 2014 - 2018Evaluation of New oncolytics Poxvirus
Molecular biology: PCR, plasmid generation, Western blot
Virology: generation, production and purification of recombinant virus
Cellular biology: viability test (oncolytic acitivity), monitoring of viral replication by titration, combination with drug
Biochemistry: enzymatic assay with HPLC
Therapeutic preclinical evaluation into murine model: sub cutaneous and orthotopic human cancer model (glioblastome, pancreatic cancer, colorectal cancer...)
Transgene
- Process Engineer - Process Development
Illkirch Graffenstaden Cedex 2013 - 2014Virus production on differents cell type (adherent, suspension)
Involvement in the establishment of a purification process
Conducting purification assay (cell breaking, clarification, enzymatic breaking, chromatography, cross flow filtration)
Writing related procedures and reports
Presentation in department meeting
Training of technicians
Process transfer to industrial development
Transgène
- Apprentice - Process Developpement
Illkirch Graffenstaden Cedex 2010 - 2013Improving virus productivity by optimization of bioprocess strategy
Cultivation of primary cells and maintenance of cell lines
Use of differents cell culture support (controlled bioreactor, cell factories, flask)
Production of viral and cell banks
Analysing related results
Writing procedures and reports
Management and organisation of the laboratory
Web conferencing with partner
Presentation at the ESACT 2013 Poster Prize session
RITTMO AgroEnvironnement
- Stagiaire Technicienne de Laboratoire
COLMAR cedex2010 - 2010Mise au point du Test d'évitement vers de terre :méthode de détection de produits toxiques dans les sols par évitement d'une population de vers de terre de la zone contaminée
Mise au point de la procédure
Fabrication du matériel de test
Maintenance des vers
Organisation des essais (préparation du matériel, mise en place des solutions et milieux)
Regulatory, legal and ethical aspects of experimental surgery
Principles of surgery and general anatomy
Anesthesia, analgesia, supervision and monitoring
Initiation into surgical procedure: Thoracic and abdominal surgery, Microsurgery