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Mireille CENTLIVRE

PARIS

En résumé

EXPERTISE
Scientific
- virology: viral genome engineering; viral stock production and quantification; viral replication kinetics; growth competition experiments;
- molecular biology: DNA and RNA extraction from tissue, faeces and cell culture; PCR; RT-PCR; quantitative (RT-)PCR (TaqMan); cloning; sequencing; gel shift assay; Northern blot;
- cell biology and immunology: culture of primary cells and cells lines; cell transduction and electroporation; thymic histoculture; 8-color flow cytometry (FACS); cell isolation from human biological material (fetal liver, blood);
- animal work: determination of in vivo SIV infection parameters (viral load, antibodies titre, CD4+ and CD8+ T cells count) in Rhesus macaque (Macaca Mulatta); cell isolation from the gastrointestinal tract (intra-epithelial lymphocytes and lamina propria lymphocytes), blood (peripheral blood mononuclear cells, macrophage); engineering of humanized mouse models (BALB/C Rag2-/-gc-/- and NOD SCID gc-/- mice) and infection of humanized mice with HIV;

Management
short-term and long-term project management; team supervision (students and technician); project reporting; redaction and reviewing of scientific articles and research grants; set-up and steering of national and international collaborations; set-up of a phase I clinical trial; treasurer (2002-03) and member of the Board of Director (2002-05) of StaPa, the association of the Pasteur Institute Young Researchers;

Language
French (mother language);
English (fluent);
German and Dutch (notions);

Mes compétences :
Cell Biology
Biologie moléculaire
Biotechnologies
Communication
Gestion de projet

Entreprises

  • Lab stratégies de vaccination et immunité, U1135, CIMI-Paris, Pitié Salpêtrière, Paris, France - Chercheur post-doctoral

    2012 - maintenant

  • Academic Medical Center of the University of Amsterdam - Chercheur post-doctoral

    2005 - 2012 Post-doctoral researcher in the Laboratory of Experimental Virology, Academic Medical Center (AMC), Amsterdam, the Netherlands; director and supervisor: Prof. B.Berkhout
    1. Development of an effective RNA interference-based anti-HIV-1 gene therapy by stable expression of HIV-1-specific short hairpin RNAs delivered by a lentiviral vector;
    2. Conditional live virus as a novel approach towards a safe live-attenuated HIV vaccine and its evaluation in the “Human Immune System” (HIS) mouse;
    3. Study of the dynamics of leukocyte cell subsets and their relative infection level during HIV infection in an acute and chronic HIV infected cohort;
    4. Delivery of biological in vivo (targeted liposomes) in the HIS mouse model;

  • BIOformation, Institut Européen de Formation Continue - Enseignant

    2003 - 2004 Molecular Biology Teacher
    BIOformation, Institut Européen de Formation Continue
    Pitié Salpêtrière Hospital, Paris, France

    Teaching for technicians, engineers and researchers: “PCR and its applications”

  • StaPa - Administratrice

    2002 - 2005 Association StaPa (Stagiaires de l'Institut Pasteur)

    Objectif:
    - Social, pour favoriser la rencontre entre stagiaires des différents départements, organiser des rencontres conviviales qui permettent à tous les stagiaires de se connaître et de s'entraider. Aider les nouveaux qui arrivent sur le campus, favoriser l'insertion et l'accueil des stagiaires étrangers.
    - Scientifique, pour favoriser le dialogue et les échanges entre stagiaires, tant scientifiques que techniques. Favoriser également le dialogue avec des chercheurs confirmés par l'organisation de manifestations scientifiques.

    www.stapa.fr

  • StaPa - Trésoriere

    2002 - 2003 Association StaPa (Stagiaires de l'Institut Pasteur)

    Objectif:
    - Social, pour favoriser la rencontre entre stagiaires des différents départements, organiser des rencontres conviviales qui permettent à tous les stagiaires de se connaître et de s'entraider. Aider les nouveaux qui arrivent sur le campus, favoriser l'insertion et l'accueil des stagiaires étrangers.
    - Scientifique, pour favoriser le dialogue et les échanges entre stagiaires, tant scientifiques que techniques. Favoriser également le dialogue avec des chercheurs confirmés par l'organisation de manifestations scientifiques.

    www.stapa.fr

  • Institut Pasteur - Chercheur doctorant

    Paris 2001 - 2005 Ph.D. scientist in Virology in the Molecular Retrovirology Unit, Pasteur Institute, Paris, France; director: Prof. S. Wain-Hobson, supervisor: Dr Monica Sala (3.5 years)
    Influence of HIV-1 subtype promoter polymorphism on viral replication dynamic in vivo
    Viral promoters modulate transcription and hence viral replication. The HIV-1 promoter is polymorph in its transcription factors binding sites. The impact of this polymorphism on viral replication in vivo has never been addressed. On the background of a SIVmac239 genome, we have engineered three chimeras bearing the minimal promoters of HIV-1 clade B, C and E. By co-infection of four rhesus macaques, a discrepancy between the genotypes of rPBMCs-associated chimeras and of virions in the serum has been highlighted. A shift in viral genotype profiles was observed between the acute and the asymptomatic phase. These results show that subtype-specific HIV-1 promoter polymorphism is a major determinant influencing viral replication and dissemination. Notably, primary infection viremia is mainly due to HIV-1 replication in the gut.
    Keywords:HIV-1, LTR, subtype, rhesus macaque, primary infection, IL-7

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