Sènan Mickaël D'ALMEIDA, PHD

En résumé

Entreprises

• Ludwig Institute for Cancer Research - Research Scientist / Post-doctoral Researcher chez Ludwig Cancer Research

  2016 - maintenant Systems immunopharmacology. Developing tools to assess the mechanism of action of combinatorial therapies and immune checkpoint blockades in cancer.

• Harvard Medical School - Mass. General Hospital - Visiting Research Fellow

  2016 - 2016 Investigating Predictive Biomarkers to improve Cancer Immunotherapy.

• Université d'Angers - Consultant

  Angers 2012 - 2013 The mission will be about consultancy for a project in nanomedicine, named Nanofar. Under the direction of Dr Franck Boury.

• Inserm - PhD student in Onco-Immunology

  PARIS 13 2012 - 2015 Working for the "Centre de Recherche en Cancérologie Nantes-Angers" (CRCNA), UMR Inserm U892 (team 7), my projects are about the biology of dendritic cells, the tumor-associated-macrophages and their implication in immunotherapy.
  
  Project 1: Immunosuppressive phenotype of tumor-associated macrophages in ovarian cancer and mesothelioma
  1) Predictive biomarkers validation for specific immunotherapy treatment response
  2) Cell-based assays involving primary cells and several cell lines (flow cytometry, ELISA, proliferation, cell death, intracellular signalling, phagocytosis, confocal microscopy, immunocytochemistry, western blot, qPCR)
  3) Anti-tumoral vaccine design (water-in-oil preparation)
  4) In vivo studies on ovarian tumoral microenvironment (1D8 mice model)
  
  
  Project 2: Immunosuppressive functions of tumor-associated macrophages in mesothelioma
  1) Evaluation of tumor cell death after chemotherapeutic treatment (alimta +/- cisplatin)
  2) Cell-based assays involving primary cells and several cell lines (flow cytometry, ELISA, proliferation, confocal microscopy, immunocytochemistry, western blot, qPCR)
  
  Project 3: Defective functions of myeloid cells in cystic fribrosis
  1) Interaction fungi / macrophages
  2) Effect of fungal infection on macrohage differentiation

• Institut Gustave Roussy - Research engineer in immunotherapy (intern) - U1015 (Pr Zitvogel)

  Villejuif 2012 - 2012 Study of anti-tumoral effect and side effects of Immunotherapy versus chemotherapy in animal models
  
  Characterization of the inflammatory effects of Ipilimumab, a monoclonal antibody (Yervoy, BMS) compared to anthracyclins (Cyclophosphamide and Doxorubicin): Tumor bed versus gut immunity
  

• UMC St Radboud (Nijmegen Center for Molecular Life Sciences), Nijmegen, Pays-Bas - Intern in Immunology/ Oncology Research

  2011 - 2012 Cytotoxic T Lymphocytes (CTLs) cell dynamics: study of the immunological synapse with cancer cells and the activation pathway.
  (Collaboration with MD Anderson Cancer Center, Houston, TX, USA)
  
  - Cell culture
  - Dynamic Imaging of CTL in cancer cells microenvironment
  - Confocal Microscopy - Immunostaining
  - Flow cytometry

• PrimaDiag - Consultant

  Romainville 2011 - 2011 ISO standards, IVD reglementation, Business plan

• Institut Sup'biotech Paris - Student in Master's degree - option R&D in health (semester abroad)

  2011 - 2012 Engineer in Biotechnology (Paris, France) and Master in Medical Biology at Radboud University (Nijmegen, The Netherlands)

• Pherecydes Pharma - Ingineer intern

  2010 - 2011 Production of bacteriophage biobank and Quality Control under the direction of Dr Pouillot F.

• Institut de cancérologie Gustave Roussy - Unité 1009: Hématopoïèse et cellules souches - Intern (Dr Vainchenker's lab)

  2010 - 2010 Studied molecular mechanisms involved in the Essential Thrombocytemia.

• Ionis tutoring - Teacher in Biology, Biochemistry, Methodology and English

  2009 - 2012 Biology at High School and "classes préparatoires" (SVT)
  Biochemistry at High School level (STL)
  English at High School level (Enseignement général de l'Anglais)

Formations

• Radboud Universiteit Nijmegen (Nijmegen)

  Nijmegen 2011 - 2012 Master in Medical Biology
  
  Track: Clinical biology
  
  Completed by an internship of 3 months in lab (immunology and cell dynamics)

• Institut Sup'Biotech De Paris ISBP (Villejuif)

  Villejuif 2009 - 2012 Biotechnologies

• Université Lille 1 Sciences Et Technologies

  Villeneuve D'Ascq 2008 - 2009 Cellular biology and physiology (licence 2è année / Bachelor)

Réseau

• Arditti LAETITIA

• Julien COMBES

• Julien SEBRIER

• Marion LEPRIEUR

• Mathilde DAURES

• Maxime CAMPOS

• Monique OLIMA

• Raffy DEMIRDJIAN

• Sébastien PERNEL

• Sylvain DUGAT