2013 - 2014In charge of NGS high-throughtput sequencing
- Illumina sequencing (MiSeq, HiSeq 2500/cBot, NextSeq 500)
- Training of national and international technical personnel to NGS sequencing
- Technological developments on Illumina NGS sequencing
- « Bead arrays » technology (iScan from Illumina)
- qPCR (LightCycler 1.5 from Roche, 7900 HT from Applied Biosystems)
- Administrative : meeting and client contact, implementation of the sequencing strategy, quote creation, projects technical monitoring, activity reports and reported results, inventory and orders management, ISO9001 and NFX 50-900 quality procedures
CNRS - ProfileXpert
- Master 2 trainee
2012 - 2013CNRS UMR 5557 - ProfileXpert, genomic and microgenomic plateform
Evaluation of a new viral genomes enrichment method (HIV-1, HIV-2, DENV, HCV, HBV, HTLV-1, HTLV-2) by capture hybridization before NGS sequencing.
- Total RNA extraction, rRNA depletion, RNA-seq libraries construction
- Viral genomes enrichment by magnetic probes and purification with magnetic beads
- LM-PCR amplification and quantification by qPCR (SYBR green)
Publication : Dupinay T, Nguyen A, Croze S, Barbet F, Rey C, Mavingui P, Pépin M, Lachuer H, Legras-Lachuer C. Next-generation sequencing of ultra-low copy samples : from clinical FFPE samples to single-cell sequencing. Curr Top Virol, 2012 ; 10 : 63-83 (http://www.researchtrends.net/tia/abstract.asp?in=0&vn=10&tid=38&aid=3839&pub=2012&type=3)
Poster : Nguyen A, Croze S, Rey C, Dupinay T, Mavingui P, Legras-Lachuer C. Development of a viral genome capture system for virus enrichment. Journée scientifique, ARC 1 Santé, 2013
INRA
- Master 1 trainee
Paris2012 - 2012INRA UMR 754
Production of the pathogenic EIAVuk (Equine Infectious Anemia Virus) viral strain by FEK, D17 and ED cell lines.
- Cell culture, cell transfection, viral particles production
- Protein assay, western-blot
- RNA extraction, RT-nPCR
Infectiologie (Virologie, Immunologie, Bactériologie), Microbiologie en terrain de laboratoire, Biologie cellulaire et moléculaire, Génétique formelle et moléculaire, Biostatistiques, Informatique pour les biologistes