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Agnès NGUYEN

LYON

En résumé

Mes compétences :
Beadarray
Séquençage Illumina
Culture cellulaire
Virologie
Génomique
QPCR, RT, nPCR, LM-PCR
Dosage protéique, Western-blot
BPL, P2, P3, Norme ISO 9001

Entreprises

  • Biofidal - NGS sequencing engineer

    2015 - maintenant In charge of NGS high-throughtput sequencing
    - Implementation and development of the Illumina MiSeq sequencing platform
    - Technological developments on Illumina NGS sequencing
    - qPCR (CFX96 Touch™ Real-Time PCR Detection System, Bio-Rad)
    - Droplet digital PCR (ddPCR – QX100™ Droplet Digital™ PCR System, Bio-Rad)
    - Additional services: nucleic acid quantitation, qualification and normalization
    - NGS project management: NGS expertise, meeting with collaborators, implementation of the sequencing strategy, quote creation, project technical monitoring, activity reports and reporting
    - Administrative: price list creation, inventory and orders management, establishment
  • ProfileXpert platform - ViroScan3D - NGS sequencing engineer

    2013 - 2014 In charge of NGS high-throughtput sequencing
    - Illumina sequencing (MiSeq, HiSeq 2500/cBot, NextSeq 500)
    - Training of national and international technical personnel to NGS sequencing
    - Technological developments on Illumina NGS sequencing
    - « Bead arrays » technology (iScan from Illumina)
    - qPCR (LightCycler 1.5 from Roche, 7900 HT from Applied Biosystems)
    - Administrative : meeting and client contact, implementation of the sequencing strategy, quote creation, projects technical monitoring, activity reports and reported results, inventory and orders management, ISO9001 and NFX 50-900 quality procedures
  • CNRS - ProfileXpert - Master 2 trainee

    2012 - 2013 CNRS UMR 5557 - ProfileXpert, genomic and microgenomic plateform

    Evaluation of a new viral genomes enrichment method (HIV-1, HIV-2, DENV, HCV, HBV, HTLV-1, HTLV-2) by capture hybridization before NGS sequencing.

    - Total RNA extraction, rRNA depletion, RNA-seq libraries construction
    - Viral genomes enrichment by magnetic probes and purification with magnetic beads
    - LM-PCR amplification and quantification by qPCR (SYBR green)

    Publication : Dupinay T, Nguyen A, Croze S, Barbet F, Rey C, Mavingui P, Pépin M, Lachuer H, Legras-Lachuer C. Next-generation sequencing of ultra-low copy samples : from clinical FFPE samples to single-cell sequencing. Curr Top Virol, 2012 ; 10 : 63-83 (http://www.researchtrends.net/tia/abstract.asp?in=0&vn=10&tid=38&aid=3839&pub=2012&type=3)

    Poster : Nguyen A, Croze S, Rey C, Dupinay T, Mavingui P, Legras-Lachuer C. Development of a viral genome capture system for virus enrichment. Journée scientifique, ARC 1 Santé, 2013
  • INRA - Master 1 trainee

    Paris 2012 - 2012 INRA UMR 754
    Production of the pathogenic EIAVuk (Equine Infectious Anemia Virus) viral strain by FEK, D17 and ED cell lines.

    - Cell culture, cell transfection, viral particles production
    - Protein assay, western-blot
    - RNA extraction, RT-nPCR

Formations

  • Université Lyon 1 Claude Bernard

    Villeurbanne 2012 - 2013 Master 2 Recherche Infectiologie Fondamentale

    Bioinformatique, Biostatistiques, Biosécurité, Virologie, Immunologie, Bactériologie
  • Université Lyon 1 Claude Bernard

    Villeurbanne 2011 - 2012 Master 1 Génétique et Biologie de la Cellule

    Virologie-Vectorologie, Immunologie et Immunopathologie, Maladies génétiques, Vie et mort cellulaire-Oncogénèse
  • Université Lyon 1 Claude Bernard

    Villeurbanne 2010 - 2011 Licence 3 Microbiologie

    Microbiologie (Biologie cellulaire microbienne, Ecologie microbienne, Bases en génétique bactérienne), Outils et bases moléculaires, Intéractions entre macromolécules, Biologie cellulaire
  • Université Paris 7 Denis Diderot

    Paris 2009 - 2010 Licence 2 Biologie-Biochimie

    Infectiologie (Virologie, Immunologie, Bactériologie), Microbiologie en terrain de laboratoire, Biologie cellulaire et moléculaire, Génétique formelle et moléculaire, Biostatistiques, Informatique pour les biologistes
  • Université Paris 7 Denis Diderot

    Paris 2008 - 2009 Licence 1 Sciences de la Nature et de la Vie

    Biologie cellulaire et moléculaire, Biostatistiques

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