-
CEA
- Lab manager
PARIS
2015 - maintenant
iRCM department of Mr P-H. Roméo, Laboratoire sur la Réparation et la Transcription dans les cellules Souches (LRTS) Repair and transcription on hematopoïetic stem cells
Active participation to those following lab projets:
- The characterization of MUSE cells and their use in several models
- Impact of TRIM 33 factor in macrophagies during induced colon acute inflammation
- Exploration of JAK2 mutation V617F in myleoproliferatif model; engraftment and following of stem cells clonal expansion between irradiated and no irradiated leg
Key words: flow cytometry (LSRII, Canto, Cyan) - cell sorting (AriaII) - cryostat - immunofluorescent staining - immunohistochemistry (IHC) - confocal and normal microscopy (SPE, SP8, Nikon A1, axio)
Laboratory management: budget, orders, stocks, lab organization, dispatches, maintenance and repear of lab equipment
-
Commissariat à l'énergie Atomique et aux énergies alternatives
- Engineer Assistant
2007 - 2015
ImmunoVirologie department of Mr R. Le Grand, laboratory Li2 (old name: TIPIV)
Lab management of the genomic platform : virus load quantification for internal and external studies, primate plateform ANRS included
- Work in biosafety level 3 laboratory on SIVmac251, SIVagmSAB92018, SHIV clade C & 162P3, Chikungunya and Dengue viruses
- DNA & RNA Extractions from macaca fascicularis and agm samples (blood, sera, plasma, tissus, cells)
- RTqPCR et qPCR (thermocylers Bio-Rad iCycler, MYiQ, CFX96)
- Work flow management, datas analysis (Prism), databases (EworkBook, BatLab), interaction with Partners
- Lab management: equipment training, maintenance, stocks, orders
- Training and supervisory of people (Trainees, Partners, GLP references for lab users)
- Participation of samples library , NFS, ELISPOT, viral production
-
CGMC UCBL UMR 5534 (CNRS)
- Lab Technician in molecular biology
2006 - 2007
CGMC UCBL UMR 5534, Molecular genetic of Simplex Herpes I Virus Laboratory directed by A. Epstein
TOLVEN project - Development of oncolytic recombinant viruses derived from Simplex Herpes I Virus to cure hepatocellular carcinoma
CompuVac project participation
- Cloning, bacterial transformation, transfection, western blot, cell culture, immunofluorescence staining, fluorescence microscopy
- Creation development and management of clonal bank
- Lab management: orders and stock for molecular biology part
- English supervisory of partners for molecular biology part
-
Sanofi Pasteur
- Lab Technician in Molecular and Cell Biology
Lyon
2005 - 2005
Research laboratory of Catherine Moste, Preclinical Translationnal Research laboratory of Véronique Barban
Validation of Dengue virus load titre in a clinical essai
Checking of the Poliomyelitis virus DNA sequence
RTqPCR (iCycler), virus titration by cytopathic effects, PCR, pyrosequencing
-
Sanofi Pasteur
- Lab Technician in Molecular Biology
Lyon
2005 - 2005
Research laboratory of Mme Catherine Moste, Department of Microbiology directed by Mme Bachra Rokbi
Validation of the Nesseria Meningitidis B MC58 DNA sequence - find new vaccinal candidates
Several differences were found between the Glimmer gene prediction software in microbial DNA of TIGR and the Prokov prediction software of INR
PCR, clone production, DNA extraction, Sanger sequencing (LicorII), sequencing analysis software (E-seq, Vector NTI) oligo design software (Oligo6, DNASTAR), agarose and acrylamide
gels
