Mes compétences :
Gestion de projet
Biotechnologies
R&D
Entreprises
Corpus Humanum
- Enseignant
2014 - 2016Ecole prépa. concours PACES.
Enseignant en Santé Publique (UE7b) et Pharmacologie (UE6) en 1ère année PACES
IOB (Bordeaux)
- Professeur
2013 - maintenantEnseignant en Physiologie générale, musculo-squelettique, téguments et organes des sens, Physiologie cardiovasculaire et respiratoire, Biologie cellulaire, Biochimie, Biologie moléculaire, Génétique, Hématologie, Immunologie, Histologie et Embryologie (1ère et 2ème année).
Alderys
- Chef du département de Génétique Moléculaire
2010 - 2012
Direction et gestion du département.
Organisation et mise en place du laboratoire.
Chef de projet.
Délétion et insertion de gènes d'intérêt, chez la levure et la bactérie.
2009 - 2010Tests MIC (G+, G-), kill curves, évaluation des fréquences de mutations spontanées.
Representant microbiologiste dans un projet préclinique.
Molecular Biology: PCR, qRT-PCR.
Cloning of gyrA, gyrB, parC and parE of S. aureus, and of beta-lactamases in expression vectors.
Directed mutagenesis (gyrA and parC of S. aureus, various beta-lactamases).
Building of vectors for gene deletions in P. aeruginosa.
Biochemistry: Expression and purification of gyrase and topoIV sub-units of S. aureus. Implementation of decatenation assay and evaluation of NXL101 activity as compared to reference cpds.
Implementation of ATPase activity assay of S. aureus gyrase. Evaluation of the activity of internal and reference cpds.
Evaluation of the beta-lactamase activity on P. aeruginosa cells and cell lysates (nitrocefin hydrolysis). Evaluation of the activity of internal and reference cpds.
HMR then Aventis
- Scientist then Senior Scientist, Project Leader
1999 - 2004Project Leader.
Antifungal Microbiologist:
Test internal cpds in vitro and in vivo (septisemia, tissue infection).
Evaluation of the acquisition of resistance to aminocandin in vitro (passages and spontaneous mutation frequency) and in vivo (passages).
Implementation of a model of C. glabrata infection in immunocompromised mice.
Evaluation of the activity of aminocandin on the tissue infection of A. fumigatus by qPCR (lungs, kidneys, brain and liver).
Institut Bergonié/INSERM collaboration
- Research Ingeneer
1996 - 1999Evaluation of the cardiotoxicity of anthracyclines using the isolated perfused rat heart model. Dosing of drugs and metabolites concentrations in the left ventricule by HPLC.
