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Elise ALBERT

AVIGNON

En résumé

PhD in plant genetics.

Genotype by environment interaction. Linkage mapping. Association mapping. RNA sequencing. Differential gene expression. Allele specific expression. Gene expression networks.

Mes compétences :
Statistiques
Phytopathologie
Agronomie
Ingénierie
SPSS Statistics
Analyse quantitative
Génétique
Biologie
GWASS

Entreprises

  • Michigan State University - Postdoctoral researcher

    2017 - maintenant Carotenoids, Vitamins E and B synthesis, Quantitative genetics and genomics.

  • INRA - PhD Student - Plant Genetics (Unit GAFL / Team QUALITom)

    Paris 2013 - 2017 ABSTRACT
    Water scarcity will constitute a crucial constraint for agricultural productivity in a near future. High throughput approaches in model species have identified hundreds of genes potentially involved in survival under drought conditions, but very few having beneficial effects on quality and yield in crops plants. Nonetheless, controlled water deficits may improve fleshy fruit quality through weaker dilution and/or accumulation of nutritional compounds. In this context, the first part of the PhD was aimed at deciphering the genetic determinants of the phenotypic response to water deficit in tomato by exploring the genotype by watering regime (G x W) and QTL by watering regime (QTL x W) interactions in two populations. The first population consisted in recombinant inbreed lines (RIL) from a cross between two cultivated accessions and the second was composed of diverse small fruit tomato accessions mostly native from South America. Plants were phenotyped for major plant and fruit quality traits and genotyped for thousands of SNP. Data were analyzed within the linkage and association mapping frameworks allowing the identification of QTLs and putative candidate genes for response to water deficit in tomato. The second part of the PhD had the objective to explore gene regulation in green fruit and leaves of tomato plants stressed by water deficit. For this purpose, RNA-Seq data were collected on the two parental genotypes of the RIL population and their F1 hybrid. Data were analyzed to identify differentially expressed genes and allele specific expression (ASE). Then, the expression of 200 genes was measured in leaves and fruits of the whole RIL population by high throughput microfluidic qPCR. eQTLs and eQTL by watering regime interactions were mapped for those genes using linkage mapping. Colocalisations with the phenotypic QTLs were analyzed. The knowledge produced during this PhD will contribute to a better understanding of the tomato plant interaction with their environment and provide bases for improvement of fruit quality under limited water supply.

  • INRA - Internship - Master 2 / Ingénieur en Agriculture

    Paris 2013 - 2013 Genetic variability of black poplar (P.nigra L.) partial resistance against Melampsora larici-populina Kleb, responsible for foliar rust. Strain by genotype interaction. Validation of QTL for partial resistance.

    Collaboration in Dr. Catherine Bastien team.

  • WAGENINGEN UNIVERSITY - Plant Reserach International - The Netherlands - Internship - Master 1

    2012 - 2012 New methods of seed ageing for primed lettuce seeds using oxygen high presured.

    Collaboration in Dr. Steven GROOT team (department Biosciences, group Plant Research International).

  • RIJK ZWAAN BV - The Netherlands - Internship - Bachelor degree

    2010 - 2010 Priming protocols for kolhrabi seeds.

    Supervisors : Ronald Van Leeuwen et Ronald Driessen

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