Mes compétences :
Cell culture
Chromatography
Culture
DNA
Downstream
Filtration
Process
Process Development
Purification
Scale up
Upstream
Entreprises
ThromboGenics
- Purification Expert & Team Leader
2009 - maintenant- Purification and analysis of monoclonal antibodies (MAbs) for pre-clinical use (1-10 g scale)
- Process development for the purification of recombinant proteins (IEX, IMAC, affinity...)
- Preclinical production Team leader: supervison of USP & DSP activities (& molecular biology)
Vivalis
- Downstream Process Development, Head
Roussay,2007 - 2009Process development for the purification of viral vaccines (MVA) and monoclonal antibodies MAbs (2-20 L scale)
- Development of purification processes: cell lysis, clarification, tangential flow filtration (TFF flat sheets and hollow fibers), ion-exchange on membrane, affinity chromatography, gel filtration (SEC), Benzonase digestion…
- Large contribution to GMP transfer (up-scaling to 100 L, equipment design, FAT)
- Study of the potential contaminants: host-cell proteins, DNA, virus clearance
- Involvement in project management with industrial partners
- Coaching of 2 engineers
Cayla - InvivoGen
- Production of recombinant proteins, Head (cell culture & purification)
Villefranche-de-Rouergue2005 - 2007Process development for the production of recombinant cytokines, antibodies (PAbs, IgA), receptors…
- Development of purification processes: ion exchange (IEX), affinity and HIC chromatography, SEC, tangential ultra-filtration…
- Stability optimization during lyophilization (addition of stabilizers)
- Development of mammalian cell culture processes: transient expression by HEK 293 cells, cell adaptation to serum-free culture (suspension), transfection optimization
- Coaching of 2 technicians
MolMed
- Pilot plant scientist for the purification of viral vectors & recombinant proteins
2002 - 2005Development of purification and culture processes for the production of viral vectors and recombinant proteins (cytokines and MAbs), set-up of analytical methods
- Development of processes for the purification of retroviral particles and recombinant proteins: TFF, SEC, HIC, IEX, affinity, RP-chromatography, precipitation, DNase treatment…
- Up-scaling to 20 L for TFF process (Pilot plant)
