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Mathilde DECOURCELLE

Paris

En résumé

Mes compétences :
Label-free
Phosphopeptides
nanoLC - MS/MS coupled

Entreprises

  • CNRS - Proteomics engineer

    Paris 2015 - maintenant UMR 5004 - BPMP (Biochemistry and Plant Molecular Physiology) - "UbiStress team" and "Mass Spectrometry Proteomics Platform"

  • Club Jeunes SFEAP - Committee member

    2013 - 2015 committee member (secretary)

    http://www.viadeo.com/groups/?containerId=00226qhblbq7y4wo

  • INRA - Proteomics engineer (mass spectrometry)

    Paris 2011 - 2014 UMR BPMP, "Mass Spectrometry Proteomics Platform", Montpellier

    European project "CaroMaize" (ANR, Plant-KBBE)

    Quantitative and comparative analyses by mass spectrometry (Label-free) of proteomes from maize seeds endosperm enriched with carotenoids.

    Methods development : fractionation by SDS-PAGE or SCX (chromatography)
    Establishment of label-free quantification
    Nano LC - MS/MS analyses
    Nano LC - MS/MS maintenance
    Data processing

  • INRA - Intership - Research engineer

    Paris 2011 - 2011 INRA, Montpellier, "Laboratoire de Protéomique Fonctionnelle" U1199

    Study of Arabidopsis thaliana phosphoproteome after iron stress
    Establishment of quantitative phosphoproteomic approaches.

    - Protein extraction
    - SCX fractionation
    - Phosphopeptides enrichment (TiO2)
    - nanoLC ESI-MS/MS analyses (Q-TOF, Bruker, and Ion Trap, Bruker)
    - Quantification by Label-free.

  • Laboratoires Biové - R&D internship

    2010 - 2010 Works on nutritional supplement for bees.
    Analyses of proteins composition

    - Ionic an reverse phase chromatographies
    - Electrophoresis (SDS-PAGE, Western Blot, Zymography)
    - Quantitative analysis (ELISA)

Formations

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