Alizé Pharma SAS
- Biopharmaceutical Development Manager
2009 - maintenantLeading and managing the bioprocess development, the analytical controls, the formulation development and any manufacturing activities related to the development of new biopharmaceutical products. Participation in the drafting and implementation of development plans. Key contact with CMOs and CROs for technical and scientific aspects. Preparation and management of the projects (schedule, cost, resources,). Preparation and/or review of the CMC sections of regulatory documents (investigators’ brochure, Briefing package, IMPD, IND, CTA).
2006 - 2006Setup of a proteomics lab. Training and supervision of scientists and .technical personnel.
Expression purification and analysis of secreted proteins from bacteria and parasites. Activity tests for proteins/enzymes (in vitro and in vivo). Interaction protein – protein. Proteomics (2D gels and mass spectrometry).
Purchasing and maintaining of different equipments of protein purification and proteomics
Transgene SA
- Head of Biochemistry Department
2006 - 2009Leading a team of 6 technicians, engineers and Ph D. Managing the biochemistry lab particularly expression purification and quality control. Reporting. Purchase and the maintenance of lab equipment in the laboratory (FPLC, HPLC, spectrophotometer, 2D gel apparatus, densitometer, capillary electrophoresis, …). Evaluation and implementation of new technologies and development of collaborations with subcontractors and other biotech and pharmaceuticals companies. Relationships with suppliers.
Implementation of a platform for protein expression in E. coli. Purification and functional and structural analysis of recombinant proteins expressed in prokaryotic and eukaryotic systems. Expression, purification, and structural analysis of monoclonal antibodies. Transfer of purification protocol to pilot unit. Engineering of recombinant antibodies (humanisation, chimeric). Analysis of cell lines and search of biomarkers by proteomic techniques (2D gels electrophoresis, DIGE technology, mass spectrometry). Quality control of proteins, antibodies, and peptides. Scientific consulting for other departments (in particular control quality department).
Avidis SA
- Project Leader
2001 - 2004Expression, purification, refolding and biophysical and functional analysis of recombinant soluble and membrane proteins expressed in E. coli. Implementation of a robotic process which permits a high throughput analysis for protein refolding. Activity test for proteins and peptides in vitro and in vivo.
Training and supervision of scientists and technicians in purification, refolding, and biophysical and functional analysis of proteins. Project management. SOP writing. Evaluation of new technologies. Relationship with suppliers. Purchase of medium scale equipment during start-up phase.
1997 - 2000Project:over-expression, purification, and functional and structural characterization of different members of the multidrug resistance protein family. These membrane transporters confer the resistance to anticancer drug by exclusion of these drugs from the targeted cancer cells. The goal was to better understand the structure and the activities of these glycoprotein transporters to synthesize chemical components able to block these transporters. For this study, these glycoproteins were over-expressed in yeast cells.
Management of technicians and PhD student.
Indiana University Bloomington, USA
- Project Leader
1995 - 1997Project: purification and reconstitution of a phospholipid transporter from the plasma membrane of erythrocytes, obtaining of monoclonal antibodies which recognize and inhibit this membrane transporter. This membrane transporter plays a major role in different steps of the blood coagulation cascade and possesses Mg-ATPase activity regulated by its phospholipid environment.
