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Pascale PEYRON-SALEK

TOURNEFEUILLE

En résumé

? Work in BSL3 conditions with pathogenic and non pathogenic organisms.

? Animal handling under BSL3 conditions, administration and sample extraction (fat, liver, spleen, lung), procedures under analgesia or anesthesia, clinical examinations.

? Expertise in cell culture, cell isolation and primary cell culture.

? Expertise in cell handling (fractioning, organelle isolation and analysis, real-time analysis, etc)

?Instrumental knowledge for cell biology applications:
- Confocal and multiphotonic microscopy. Sample preparation for electronic microscopy.
- FACS (Fluorescence-activated cell sorting).
- Gold Beads synthesis (for SEM), coupling to rhodamin for fluorescence analysis
- Spectrofluorimetry and FRET analysis

? RNA and DNA isolation, PCR, qPCR (taqman), Western Blot, and Northern blot, Real Time PCR and Micro-array.
? Protein extraction and quantification; enzymatic assay.
? Lipid biochemistry (extraction, purification, liposomal synthesis, quantification)
? Several types of chromatography

? Co-inventor of the patent application: « Implication des ATPases de type P dans la virulence bactérienne » (patent application)
? Co-author of 17 scientific publications in peer-reviewed
? Several oral and written communications in international congresses.

? 2007-2010: Member of the Scientific Action Committee for the MMIN department (IPBS-CNRS).
? 1998-2000: Member of the advice of school and the pedagogic commission of the doctoral school.
? 1997-2000: Active member of the Association Alpha T, association of the PhD students in biology and health of Toulouse. Co-organizer of the “Bioforum Toulouse 1998” and member of the steering committee of the “3ème Carrefour Français des Biotechnologiesi

Mes compétences :
Imagerie cellulaire

Entreprises

  • CNRS-LBME - Ingénieur de recherche microscopie champs large

    2011 - maintenant In the Carpoussis Group.

  • IPBS-CNRS Toulouse - Senior researcher

    2008 - 2010 I was in the Dr Olivier Neyrolles group (Institut de Pharmacologie et de Biologie Structurale (IPBS-Centre National de la Recherche Scientifique; Toulouse), member of the Molecular Mechanisms of Mycobacterial Infections department. I investigated the role of Zinc during the infection of human macrophages by the etiologic pathogens for Tuberculosis, Mycobacterium tuberculosis (M.tb). In parallel, I validated adipocytes as a good cell model to study M.tb dormancy and made a broad assessment for therapeutic effects of a combination of novel antibiotics.Under this position, I was in charge of managing a doctorate student and I initiated collaboration with the laboratory of Dr Chantal De Chastelier.

    2011 - Botella H., Peyron P, Poincloux R…. and O. Neyrolles. P1-type ATPases mediate microbial resistance to zinc poisoning in human macrophages". Cell Host and Microbes.

    2010 - Chongzhen Wang, Pascale Peyron, Olga Mestre, Gilla Kaplan, Brigitte Gicquel & Olivier Neyrolles. Innate immune response to Mycobacterium tuberculosis W-Beijing and other genotypes. PLoS ONE. Jun;54(6):2712-5.

    2010 - Filippini P, Iona E, Piccaro G, Peyron P, Neyrolles O, Fattorini L. Activity of drug combinations against dormant Mycobacterium tuberculosis. Antimicrob Agents Chemother.Jun;54(6):2712-5.

  • CNRS - IPBS (Toulouse) - Senior researcher

    2005 - 2008 Senior researcher in Dr Frédéric Altare’s group (IPBS-Toulouse: Molecular Mechanisms of Mycobacterial Infections department). Beside my main research project, I was in charge of building up and managing three major strategic collaborations (3), including the securing of financial funds With an in vitro model of M.tb human granulomas set-up in the laboratory, I described the role and function of Foamy Macrophages in the containment of M.tb and the function of Langhans giant cells (4-5). In collaboration with Pr B. Marchou (Service des Maladies Infectieuses et Tropicales, Hôpital Purpan-Toulouse) I analyzed in vitro granulomas formation with PBMC isolated from patients with different clinical form of Tuberculosis (6) and in collaboration with Dr G. Carcelain (Service d’Immunologie cellulaire et tissulaire, INSERM U543, Hôpital de la Pitié-Salpêtrière - Paris), I characterized in vitro the immunological response in M.tb human granulomas obtained with cell from co-infected patients with VIH and Tuberculosis. During this period I managed three Master students and one technician.

    2008- P. Peyron, J. Vaubourgeix, Y. Poquet, F.Levillain, C. Botanch, F. Bardou, M. Daffé, JF Emile, B. Marchou, PJ Cardona, C. de Chastellier and F. Altare. Foamy Macrophages from Tuberculous Patients’ Granulomas Constitute a Nutrient-rich Reservoir for M. tuberculosis persistence. PLOS Pathogen. Nov;4(11):e1000204

    2008- P. Peyron, M.P. Puissegur, A. Godel, G. Lay, C Botanch, B. Payre, C. Caratero, P. Massip, B. Marchou and F. Altare. PPD skin test and Mycobacterium tuberculosis granulomas: how to improve diagnosis of Mycobacterium tuberculosis. The Open Pathology Journal. 2, 53-56.

    2007- Lay G., Poquet Y., Botanch C., P. Peyron, Bon H., Duteyrat JL. and Altare F. M.tuberculosis triggers macrophages fusion into non-phagocytic Langhans giant cells. Journal of Pathology. Nov 17. 211(1):76-85.

  • EMBL - Junior Researcher

    2001 - 2004 in Dr Gareth Griffiths’ group (Cell Biology and Cell Biophysics Program; European Molecular Biology Laboratory (EMBL) - Heidelberg, Germany). The group was devoted to investigating phagosomal cell biology. My main project was to analyzed membrane events that regulate fusion of phagosome with lysosomes in macrophages (7-10). For this reason, I learned confocal and electron microscopy and perfected myself in cell handling with organelles purification. During this period, I taught the “EMBO practical course: Modern Methods in cell Biology” ”, and I was also responsible for a Master student.

  • IPBS-CNRS Toulouse - Junior Researcher and Junior Assistant Professor

    1997 - 2000 PhD student, Paul Sabatier University, Toulouse, France. My research involved the convergence between the fields of biochemistry and cell biology in order to understand different perspectives of the infection of human monocytes by M.tb. I studied membrane fusion processes in vitro between phagosomes and lysosomes in neutrophils, and their inhibition induced by pathogenic mycobacteria. I showed the involvement of tyrosine kinases of the Src-family in this membrane fusion process (11). Furthermore, while studying the receptors involved during the entry of mycobacteria into human neutrophils, I demonstrated that Complement Receptor 3 associated to GPI anchored proteins located in rafts is responsible for the phagocytosis of M. kansasii. (12-13).
    This work was supervised by Pr T. Stegmann and Dr I. Maridonneau-Parini (Centre National de la Recherche Scientifique, IPBS-CNRS, Toulouse France)

    Junior Assistant Professor at Paul Sabatier University. Teaching activities: Biochemistry and Cell Biology (1st, 2nd, and 3rd University levels, approximately 450 h).


    2001 – P. Peyron, I. Maridonneau-Parini and T. Stegmann. Fusion of human neutrophils phagosomes with lysosomes in vitro : involvement of tyrosine kinases of the Src-family and inhibition by Mycobacteria. J. Biol. Chem. 276(38):35512-7

    2000 – P. Peyron, C. Bordier, E.-N. N’Diaye and I. Maridonneau-Parini. Phagocytosis of Mycobacterium kansasii by human neutrophils is mediated by CR3 associated to GPI anchored proteins located in rafts. Journal of Immunology. 165 (9):5186-91.

    1999 – C. Miossec-Bartoli, L. Pilastre, P. Peyron, E.-N. N’Diaye, V. Collart-Dutilleul, I. Maridonneau-Parini and A. Diu-Hercend. The new ketolide HMR3647 accumulates in the azurophil granules of human polymorphonuclear cells. Antimicrobial Agent and Chemotherapy. 43(10); 2457-2462.

  • IPBS-CNRS Toulouse - Master Degree student

    1996 - 1997 D.E.A. de Biologie et Génétique moléculaire et cellulaire - Biotechnologies.
    Option Biochimie. (UPS, Toulouse ; Mention Bien)

    IPBS-Centre National de la Recherche Scientifique, Toulouse, France.
    Two rotations of 4 months: biophysics of lipidic bilayers and Structural Biochemistry (14-15).

    1999 – F. Dumas, M.-C. Lebrun, P. Peyron, A. Lopez, JF. Tocanne. The transmembrane protein bacterioopsin affects the polarity of the hydrophobic core of the host lipid bilayer. BBA. 1421(2):295-305.

    1998 – C. Raynaud, G. Etienne, P. Peyron, M.-A. Lanéèlle and M. Daffe. Extracellular enzyme activities potentially involved in the pathogenicitiy of Mycobacterium tuberculosis. Microbiology. 144 (Pt 2): 577-587.

  • Faculté de pharmacie de Toulouse - Trainee researcher

    1995 - 1996 supervised by Dr F. Legaillard (Claudius Regaud Center and Pharmacia University-Toulouse). I was charged of the preparation of supramolecular biovectors (SMBV) (16).

    1998 - P. Picault, F Courbon, P. Peyron, T. Al Saati, R. Dirson, R. Kravtzoff, G. Delsol, G. Favre and F. Le Gaillard. Targetting of CEM lymphoma tumour cells with supramolecular biovectors (SMBVs) directed by a monoclonal antibody. Tumour targetting. 3 : 13-20.

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