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Pierre WALLET

LYON

En résumé

I have a PhD in immunology obtained in the international center for infectiology research (CIRI) in Lyon (France).

During my studies, I have decided to do an internship (6 months) in Thomas Henry’s lab where I’ve worked on Staphylococcus aureus leucotoxins. The purpose of my work was to define the host receptor for a bicomponent Staphylococcal pore forming toxin. I set up a screen in HEK293T cells transfected with a G protein-coupled receptors library and treated with different recombinant bicomponent toxins of Staphylococcus aureus. This work led to the discovery of a new receptor (CCR2) for the toxin gamma-haemolysin AB and explained why S.aureus toxins are lethal to phagocytes.

Then I started my PhD in Thomas Henry’s lab where, this time, I focus on the role of interferon-stimulated genes (ISGs) in Aim2 inflammasome activation during Francisella novicida infection. I performed a siRNA screen in primary bone marrow derived macrophages, and discovered that Guanylate binding protein (GBPs) are involved. This finding resulted in a successful collaboration with Petr Broz’s lab in Basel. Together we demonstrated for the first time that GBPs control cytosolic Francisella novicida replication by directly targeting the bacteria. Following the publication of this work in Nature immunology, I wrote 1 review in a French journal and a book chapter on Francisella interplay with the inflammasome.
I continued to characterize the newly pathway we described . This time we collaborated with different labs -one in Sweden (Anders Sjöstedt) and one in France (Max Maurin)- to investigate our results with other highly pathogenic strains. These collaborations should lead to a new publication in PLOS pathogens (paper in submission).

I wish to pursue my career in basic research but still keeping in mind the potential applications. That's why i'm looking for a post-doc in an international company. I am really interested in innate immune responses.

Mes compétences :
Travail en équipe
Rigueur
Adaptabilité
Immunologie
Biologie moléculaire
Immunofluorescence
Microbiologie
Expérimentation animale

Entreprises

  • City of Hope Los Angeles California - Staff Scientist/Post Doc

    2017 - maintenant I am working in the interaction between cancer cells and immune cells. My mission is to understand how cancer cells are able to avoid the immune system in order to proliferate but mostly if it is possible to revert this phenomenon!

  • Inserm - PhD in immunology interaction host-pathogen

    PARIS 13 2014 - 2017 The objective of my thesis was to find the link between interferon and inflammasome activation during infection with a cytosolic bacteria Francisella novicida.

    I started my PhD in Thomas Henry’s lab where I focus on the role of interferon-stimulated genes (ISGs) in AIM2 inflammasome activation during Francisella novicida infection.

    I performed a siRNA screen targeting 483 ISGs in primary bone marrow derived macrophages, and discovered that Guanylate binding protein (GBPs) 2 and 5 are involved in AIM2 inflammasome activation. This finding resulted in a successful collaboration with Petr Broz’s lab in Basel. Together we demonstrated for the first time that GBPs control cytosolic Francisella novicida replication by directly targeting the bacteria.

    Following the publication of this work in Nature Immunolgy "Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida", I wrote 1 review in a French journal (Guanylate binding proteins: new proteins from the cell host cytosol involved in antibacterial immunity. Med Sci) and a book chapter on Francisella interplay with the inflammasome. (Francisella inflammasomes: Integrated responses to a cytosolic stealth bacterium. Current Topics in Microbiology and Immunology)

    I continued to characterize the newly pathway we described. This time we collaborated with different labs -one in Sweden (Anders Sjöstedt) and one in France (Max Maurin)- to investigate our results with other highly pathogenic strains. These collaborations should lead to a new publication in PLOS pathogens (paper in submission).

  • Inserm - Internship in immunolgy host-pathogen interactions

    PARIS 13 2013 - 2013 I have decided to do an internship (6 months) in Thomas Henry’s lab where I’ve worked on Staphylococcus aureus leucotoxins.

    The purpose of my work was to define the host receptor for a bicomponent Staphylococcal pore forming toxin. I set up a screen in HEK293T cells transfected with a G protein-coupled receptors library and treated with different recombinant bicomponent toxins of Staphylococcus aureus.

    This work led to the discovery of a new receptor (CCR2) for the toxin gamma-haemolysin AB and explained why S.aureus toxins are lethal to phagocytes. "The staphylococcal toxins gamma-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors." Nat Commun

Formations

  • Université Lyon 1 Claude Bernard

    Villeurbanne 2011 - 2013 Master Recherche Ecologie Microbienne

    Master 1 Ecosciences, Microbiologie
    Master 2 Recherche Ecologie Microbienne

  • Université Lyon 1 Claude Bernard

    Villeurbanne 2008 - 2011 Licence Sciences, Technologies et santé mention biologie

    Licence Sciences, Technologies et Santé mention biologie - Disciplines : Microbiologie , Physiologie, Biologie cellulaire, génétique
    Mention assez bien

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