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Sébastien RIGO

RUEIL MALMAISON

En résumé

I'm always looking forward of new experiences in order to diversify my scientific knowledge.


Technical skills:
Molecular biology : Cloning using different technics (Gateway, pcDNAd, gene extraction from mRN A, point mutation generation), RNA isolation, cDNA synthesis, qPCR using Taqman probes and SYBR green technology
Cell biology : Culturing of primary endothelial cells, transfection using Amaxa technology.
Isolation of Monoctes and Neutrophils from blood (BL2 work)
Electrical Cell Impedance Sensing (APPLIED BIOPHYSICS)
ICC using confocal microscope (ZEISS), quantification program/ 3D images (IMARIS)
Angiogenesis assays, migration assays, proliferation/viability assays.
ELISA, FLIPR, HTRF, Luciferase reporter gene, Western blot and quantification with Ettan DIGE.
FACS analyser and sorting (PARTEC)
Aorta ring Ex vivo experiment and organ sampling for gene expression analyses
IHC staining methods

Mes compétences :
Biology
Research

Entreprises

  • Novartis - Scientific associate

    RUEIL MALMAISON 2012 - maintenant Immunology, Focus on M1/M2 polarization of macrophages
    Isolation of monocytes and neutrophils from fresh blood.
    Characterization of each preparation by FACS and staining (Cytospin).
    Support for gene signature identification of M1/M2 type of human macrophages.
    Set up of efferocytosis functional assay using FACS technology for compound profiling and target identification.

  • NOVARTIS - Scientific associate

    RUEIL MALMAISON 2007 - 2011 Endothelial biology. GPCR involved in the endothelium.
    Generation and characterization of stable cell lines.
    Development and optimization of cellular assay.
    Primary endothelial cells isolation and characterization by FACS
    Characterization of endothelial cells markers in mouse Embryonic Bodies.
    Good background in ex vivo experiment and organ sampling (mouse)
    Cloning of GPCRs and gene expression analyses of different in vivo models
    Presentation of results to journal club and poster session

  • ARPIDA - Scientist/Biochemistry Lab

    2002 - 2007 Job focus:
    Assay set up, development and optimization. Prokaryotic cell based assays and enzymatic cell free system. Protein purification
    Biology support of chemistry program.
    Writing of final reports, summaries of conferences, presentation of results to Biology/Chemistry groups and to Scientific Advisory Board members.

    Technical skills:
    Spectrophotometer/Luminescence/Flashplate/SPA based ass ays.(3H, 14C). Protein purification using His-tag. Enzyme kinetics.

Formations

Pas de formation renseignée

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