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Stalhberger THOMAS

PARIS

En résumé

Specialist in Glycobiology, my research interests include Microbiology, Biochemistry, Immunology link to skin microbiota, applications of all above. I seek to join a company with a strong focus on R&D, that will provide me with an opportunity to exploit my scientific expertise, while promoting my career and the growth of the company

During my career I have been able to acquire:
•A unique combination of academic and industrial scientific experience
•A multicultural sensitivity with experience in England.
•A wide range of scientific technical skills
•Motivated and Visionary, transmitting enthusiasm, goals, and leadership to a research team or organization. Good communication skills

Mes compétences :
Dermatology
Microscopy
Western Blotting
Microbiology
Immunology
Flow Cytometry
ELISA
lipids analysis
cell membrane analysis
Microsoft Office
DNA extraction

Entreprises

  • L'Oréal - Ingénieur de recherche

    PARIS 2014 - maintenant Development of evaluate/quantification model on Fungi of the Scalp Management for the development of Specific antibodies
    • Flow cytometrie analysis
    • Immuno-labeling
    • Evaluation model of anti-adhesion on reconstructed skin
    • Secondary metabolite analysis
    • Writing procedures (quality Format)
    • Train staff to cell culture
    • Manage internal collaboration.
    • Council / Expertise Biochemical / Fungal Microbiology / Malassezia for transversal projects
    Referees:
    Roland Jourdain, OPEN RESEARCH DEPARTMENT,L'Oréal Advanced Research
    1, avenue Eugène Schueller, 93600 Aulnay sous-bois. France
    Email: rjourdain@rd.loreal.com , Tel : +33 (0)1 48 68 96 28

    Florence Ménard-Szczebara PhD, MICROBIOLOGY CORPORATE, L’Oréal Applied Research R&I
    185 rue Paul Hochart - 94550Chevilly-Larue – France
    Email: fmenard-szczebara@rd.loreal.com, Tel: +33(0)149795108

  • Institut Pasteur - Doctorant

    Paris 2010 - 2013 Laboratory:Unité des Aspergillus

    My project is focused on the study of the cell wall organization to the yeast Malassezia restricta that is the most prominent yeast found on the human scalp.

    Skills acquired:

    Microbiology:
    •Culture of fungi/ bacteria: E. coli, S. platensis, S. epidermidis, P. acnes, P. pastoris, A. fumigatus and culture optimization and conservation for M. restricta and other Malassezia species
    •Coculture of bacteria/fungi: Development of synthetic medium for M. restricta/ P. acnes/ S. epidermidis.
    Electronic Microscopy:
    •Scanning electron microscopy: Fixation and embedding optimization for M. restricta and P. acnes
    •Transmission electron microscopy: Samples preparation for M. restricta and P. acnes
    Biochemistry:
    •Fungal cell wall/cell membrane analysis: Polysaccharides/lipids analysis by chemical and enzymatic approachs
    •Enzymology: kinetics and inhibition assays, lipid peroxide detection.
    •Separation techniques for proteins, lipids and sugars: chromatography techniques such as affinity, size exclusion, ion-exchange, thin-layer (TLC), gas or liquid chromatography coupled to mass spectrometry ; electrophoresis
    •Analytical techniques: UV-visible spectroscopy, circular dichroism, micro-calorimetry. Flow cytometry setup for clinical samples (BactiFlow), cell hydrophobicity (Goniometer, MATHS test)
    •Protein purification in Pichia pastoris and E.coli
    •Immunology: Culture of monocyte (ThP1), cytokine assays

    Referee:
    Prof. Jean Paul Latgé, Institut Pasteur - 25 rue du Docteur Roux - 75015 Paris - France
    Email: jplatge@pasteur.fr, Ttel: +33(0)140616461

  • CEA /IBiTecS/SB2SM/LBSR, (Saclay) - Stagiaire M2

    2010 - 2010 Laboratoire de Biologie Structurale et Radiobiologie:

    Study of the MLH1 protein, a protein associated with hereditary colon cancer

    Skills acquired:

    •Production and purification of proteins,
    •Interaction characterization by microcalorimetry,
    •Crystallography
    •Molecular modelisation.

  • Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, (Paris 5 ) - Stagiaire M1

    2009 - 2009 Biotransformation chez Streptomyces platensis:
    Technique abordées:
    Synthèse chimique d'un antihistaminique, la fexofénadine
    Expression en fermenteur de levures Streptomyces platensis

  • Faculty of Medicine and Human Sciences, (Manchester, UK) - Stagiaire

    2008 - 2009 University of Manchester, Wythenshawe Hospital, Manchester, UK (8 months): Cell-wall genes knock out in Aspergillus fumigatus.
    Molecular biology basic techniques (PCR, RT-PCR, DNA extraction, gene deletion), Transformation in A. fumigatus.

    Publication (Harrison, E, Stahlberger, T, & al, 2010

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