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Steve REYNAUD

Gif-sur-Yvette

En résumé

Thanks to millions of years of selective pressure, nature can provide molecules with high affinity and selectivity for their molecular target. Animal toxins and alkaloids are perfect examples of this as they can be used as pharmacological tools or even modified to make innovative drugs.

My main skills :
Peptide chemistry:
Solid-Phase Peptide synthesis, directed disulfide bridge formation, reverse-phase HPLC, mass spectrometry (MALDI-TOF, ESI-TOF), LC-MS, spectrophotometer, circular dichroism,

Pharmacology :
Binding assay using a radioligand, Functional cAMP assay, High Throughput screening, Two electrode voltage clamp

Cell culture (CHO, HEK cells):
transfection, stable cell line obtention

I am currently in a PhD fellowship in the CEA in France. I am studying animal toxins active on different G-protein coupled receptors involved in eating disorders and melanoma.

Mes compétences :
Genetics
Langage Python
HPLC
Langage R
Spectrométrie de masse

Entreprises

  • CEA Saclay - Intern

    Gif-sur-Yvette 2016 - 2016 Hits confirmation (Venomics Project) : toxins as drug candidates
    Methods:
    Peptide production and purification: reverse-phase HPLC, mass spectrometry (MALDI-TOF, ESI-IT), LC-MS, spectrophotometer

    Affinity and selectivity studies: Binding assay using a radioligand, cAMP assay See less
  • Institute for Molecular Bioscience, The University of Queensland - Intern

    2015 - 2015 Defining the binding site of Mambalgin on ASIC1a and understanding the molecular basis for the difference in potency against rat and human channel
    Mambalgins are venom peptides that potently inhibit some subtypes of Acid-Sensing Ion Channel (ASIC) and have promising analgesic activity in rodent models of pain
    Techniques and Skills:
    - Recombinant peptide production and purification : Bacterial culture expression, affinity chromatography, HPLC, mass spectrometry MALDI-TOF, ESI)
    - Ion channel mutagenisis, expression and characterisation. (primer design, quick change mutagenesis, in vitro RNA production)
    - Electrophysiological assessment of the inhibitory activity of peptides using two-electrode voltage clamp on Xenopus oocytes
  • Institut de Pharmacologie Moléculaire et Cellulaire, CNRS - Intern

    2014 - 2014 Pharmacological characterization of ASIC ion channels thanks to animal toxins and marine sponge alkaloids

    The activation of Acid Sensing Ion Channels (ASIC) by acidosis and their expression in sensory neurons makes them very interesting novel pain targets. In pursuit of this aim, animal toxins and natural alkaloids could be exellent pharmacological tools.

    - Peptide purification : RP-HPLC, cation exchange chromatography, mass spectrometer (MALDI-TOF)

    - Toxin activity : Two-electrode voltage clamp on Xenopus oocyte (Roboinject and Roboocyte)
  • Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille, CNRS - Intern

    2013 - 2013 Study of inter individual venom variation of Dendroaspis viridis using UHPLC/MALDI-TOF-TOF

    - Qualitative and Quantitative study : UHPLC/MALDI-TOF mass spectrometer

    - Result analysis : Excel matrix
  • Reptilarium du Larzac, Saint-Eulalie de Cernon - Animal keeper

    2012 - 2012 Animals : pythons, boas, monitor lizards, turtles, iguanas, alligator

    - Guided tour
    - Animal care
    - Feeding

Formations

  • Université Paris-Saclay

    Saclay 2016 - maintenant Thèse

    Animal toxins as innovative ligands for G Protein-coupled Receptor (GPCR)
    Aims :
    Following High troughput screening, production and characterization of the first animal toxins active on melanocortin 4 receptor (a GPCR expressed in hypothalamus)
    Optimization of the best hit as a potential lead in eating disorders: easy and efficient production, affinity and selectivity improvement

    Methods :
    Pept
  • Polytech'Nice-Sophia

    Sophia Antipolis 2013 - maintenant
  • Université Toulouse 3 Paul Sabatier

    Toulouse 2011 - 2013 Licence générale Biologie et Environnement parcours Biologie des Organismes, des Populations et des Ecosystèmes

    Equivalent to a three-year university degree in Ecology and Evolutionary Biology
  • Université Clermont 1 Auvergne

    Clermont Ferrand 2009 - 2011 DUT Génie Biologique option Agronomie

    Equivalent to a two-year university degree in technology in Biological Engineering

Réseau

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