Tanesha NAIKEN

En résumé

Entreprises

• C3M - Ingenieur de Recherche

  Waterbeach 2014 - 2016

• Dr Robert Ballotti - Research Scientist

  2014 - maintenant the group of Dr Robert Ballotti. INSERM 1065, C3M, Nice France. The aim of my work was to
  determine the role of HACE1 in melanoma development. I assessed melanoma cell viability, migration, adhesion
  and proliferation when the target gene was modified (overexpression or invalidation). Using Immunoprecipitation
  and Immunoblot techniques, the mechanisms and interactions involved were studied. Through Immunostaining
  (IHC and IF), I determined the localization of HACE1 and its co-localization with other proteins using a Leica
  microscope. Furthermore, in vivo experiments allowed me to follow the fate of these modified melanomas cells in
  "nude" mice using imaging techniques (biospace). Mice lung sections were visualized using a confocal microscope
  (Nikon).

• Dr Jacques Pouysségur - Student

  2010 - 2014 Ph.D student in the group of Dr Jacques Pouysségur under the supervision of Dr Cercina Onesto. UMR 7284,
  I explored the cell metabolism reprogramming in polarized macrophages by focusing on the
  potential metabolic plasticity of macrophages once in a defined activation state. Using different techniques
  (ELISA,Westen Blot, RT-PCR, Immunohistochemistry), I confirmed the phenotype of cells, when and where the
  specific macrophages' biomarkers were expressed within the tumor microenvironment, the co-localization of
  metabolic biomarkers within the macrophage phenotype. Moreover, I was able to demonstrate some metabolic
  plasticity within these polarized macrophages (sea-horse) exist by the targeting of glycolysis through the blockade
  of downstream lactic acid export catalyzed by monocarboxylate transporters. By manipulating the metabolic
  features of polarized macrophages, we observed that functional polarization of macrophages could be affected by
  changes in cell metabolism.
  
  OTHER TRAININGS AREA OF EXPERTISE

Formations

• IRCAN

  Nice 2013 - 2014 D fellowship
  
  Ph.D fellowship ``Fondation Arc pour la recherche sur le Cancer''

• IRCAN

  Nice 2010 - 2014 PhD
  
  These : Explorer la corperation metabolique entre les Macrophages associés aux tumeurs et les cellules tumorales

• Université Nice Sophia Antipolis

  Nice 2010 - 2014 Graduate Student
  
  France UMR 7284, IRCAN group of Dr Jacques Pouysségur.

• Université De Technologie De Compiègne (UTC)

  Compiegne 2007 - 2010 Masters
  
  Option : Biotechnologie

Réseau

Pas de contact professionnel