Mes compétences :
- Scientific and Technical project officer / Chargé de projet scientifique et technique
Maisons-Alfort 2015 - maintenantUnder the responsibility of the unit's leader, I have supported the global research project "Improving the diagnosis and development of an infectious model pour CEM (contagious equine metritis) ."
The objective is to develop a culture medium for improving the selective growth of the pathogen T. equigenitalis against equine genital microbiota. This results in the study of the growth of T. equigenitalis in differents media or in the presence of equine cell lines and the characterization of the needed compounds for Taylorella growth.
Scientific and technical realization of the project is under quality assurance and in the National and European Reference Laboratory ( NRL and EU-RL ) for CEM and dourine .
- PhD student
Paris2011 - 2014To characterize molecular mechanisms underlying adhesion of commensal bacteria, we used Streptococcus salivarius (SSAL) as a model. SSAL is among the most important pioneer colonizers of neonatal oral mucosal surfaces, and later becomes a predominant component of the human adult oral microbiota with pre-eminent ecological role. We developed a method to identified, through phenotypic screening assays, genes involved in SSAL adhesion to host or bacterial surfaces. In particular, we showed that the SecA2Y2 system, which comprises genes devoted to glycosylation and export of surface Serine Rich Repeat Proteins (SRRPs), participates to bacterial aggregation, biofilm formation, in vitro adhesion and colonization of mice. While all bacteria containing a similar system possess only one SRRP, the SSAL secA2Y2 locus comprises three SRRPs with complementary role in line with the previous phenotypes. Interestingly, SrpB is specifically involved in the binding to epithelial cells, while SrpC to the extracellular matrix and mucus proteins. We showed that these interactions require glycosylation of both bacterial SRPs and host surfaces. Surprisingly, we demonstrated that this essential process is shared by glycosyltransferases located in other genomic regions. This work is the first report showing the presence in a bacterium of three SRPs, which display complementary roles in bacterial-host interaction. While the SecA2Y2 system is mostly associated to virulence in pathogenic bacteria, it appears to be involved in the expression of commensal traits in SSAL, such as its colonization and its resilience to oral and intestinal niches. This work may offer new insights into the mechanisms of niche establishment (host, microbial communities) of commensal bacteria.
- Intership ingeneer
Paris2010 - 2011Streptococcus salivarius is one of the earliest colonizers of human oral cavity and gut, and may therefore contributes to immune homeostasis establishment and the regulation of host inflammatory response. This study focused on the modulatory effect of S. salivarius on intestinal epithelial cells. Three strains were screened for NF-kB, PPARγ, AP-1 modulation on reporter cell line.
Paris2010 - 2010Identify innovative texturing agent from bacteria of improving the consistency and creaminess of the finished product.
Paris2009 - 2009Detection and Characterization of surface proteins of probiotics lactobacilli. Laboratoire QAIEA / Danisco – Caen (14).