Courbevoie
BUSINESS SEGMENTS :
- Fundamental and applied research : R&D
- Manufacturing operations : Pharmaceutical Industry
PERSONAL QUALITIES :
_Proactive, results-driven and team oriented
_Good communication and interpersonal skills
_Conscientious, pragmatic and responsible
_Team management and continual improvement
TECHNICAL COMPETENCES :
Analytical Biochemistry - Proteomic:
_ GC-MS (Gas Chromatography – Mass Spectrometry) and LC-MS (Liquid Chromatography)-Shimadzu
_ Compounds extraction from biological materials (terpenes, antioxidants, bioactive compounds)
_ Absorbance and fluorescence spectrophotometry, luminescence (UV, IR) - Varian, PerkinElmer
_ Enzymatic characterization (specific activity determination, enzymatic tests, redox potential, pKa)-GraphPad
_ Electrophoresis (SDS-PAGE, Coomassie, 2D, WB) - Licor
_ Thiol chemistry (disulfide formation, reduction, isomerisation)
Assay and Purification:
_ High-performance liquid chromatography (HPLC) - Shimadzu
_ Chromatography (ion exchange, affinity, hydrophobic, size exclusion)
_ Ammonium sulfate precipitation, dialyze, crystallisation, freeze-drying, distillation, filtration, evaporation
Molecular, Cellular and µBiology:
_ Cell culture (suspension, adherent)
_ Cloning (PCR and transformation), site-directed mutagenesis
_ Fungi and Bacteria transformation (heat shock, electroporation, protoplast)
_ Recombinant proteins production (E. coli) and purification
_ DNA and RNA extraction, RT-PCR
Mes compétences :
Analytical techniques development
Team management
Proactive, results-driven and team oriented
Validation and/or analytical transfer
Management
Engineering
HPLC
Purification et identification de molécules b
Production protéines recombinantes
Mass Spectrometry
Séparation et purification
Chromatographie
Biologie moléculaire
Microbiologie
Enzymologie (cinétique, mécanisme)
Clonage, transformation
Biochimie analytique
GMP
ICH
Molecular biology
R
