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Julien SAHIRI

HUNINGUE

En résumé

Main skills:

Stimulation of primary immune cells, multicolor readout based on flow cytometry, Functional assay, CRISPR technology, mRNA in vitro transcription for T-cell electroporation and cytotoxic assay.

Description :

Master's degree in cellular and molecular engineering with over 2 years of experience in a pharmaceutical company.
Strong expertise in immunology based on in vitro methods to work with human healthy samples (cells/ blood) and mammalian cell lines.
Effective, flexible, eager to learn/improve skills and able to work with minimal supervision to support scientific projects.



https://www.visualcv.com/sahiriju

Mes compétences :
Culture cellulaire
Screening
Cartographie
Clonage
Mutagenèse
gene reporter assay
PBMC isolation
SDS PAGE
Lymphocytes T
siRNA
Western Blot
FACS
Antibodies
RNAm IVT synthesis and transfection
Primary cells
CRISPR technology

Entreprises

  • 4-Antibody ( AGENUS) - Research associate

    2014 - maintenant T-cell Immunology Unit:



    • Isolate cells and establish primary cell culture from human peripheral blood, MACS isolation, maintain in vitro cell cultures and stimulation of immune cells.
    • Perform multicolor flow cytometry-based phenotypic and functional analyses
    (FACS ARIA III: Advanced BD FACS AriaTM Operator Course, Calibur, Canto, Guava)
    • Develop and perform assays to evaluate the effect of candidate immunotherapies upon human immune cell function (Antibody binding, antibody mediated tumor cell killing Cytotoxic assay, proliferation and cytokine measurement).
    • CRISPR in cell lines and human primary T cells, DNA/RNA extraction, PCR, cloning, gene reporter assay, mRNA IVT synthesis, transfection, transduction, electroporation (Amaxa 4D nucleofector and Neon transfection system)

  • Novartis - Stage de fin d'étude

    RUEIL MALMAISON 2014 - 2014 Department Oncology, Drug discovery (volker_matthias.stucke@roche.com)

    • Mammalian Cell Culture, proliferation assays under pharmacological treatments, siRNA technology: transfection and functional analysis, qRT-PCR analysis
    • Molecular biology (Gateway cloning, PCR, Bacterial transformation, DNA/RNA preparation, site directed mutagenesis, DNA sequencing analysis)
    • Transient transfections, Cell lysis preparation, SDS-PAGE Western Blotting, cytoplasmic and nuclear fractionation, G-lisa RAC1/CDC42/RhoA Activation assay, Luciferase based gene reporter assay, Signalling pathway analysis

  • Carrefour - Préparateur de commande/livraison

    Massy 2013 - 2013

  • University of Dundee Gene Regulation Expression - Stagiaire Master 1

    2013 - 2013 Wellcome trust center of Gene Regulation and Expression
    University of Dundee (Dundee, Scotland)- Internship

    Anton Gartner Lab: Research on Cancer and Parkinson
    (b.u.wang@dundee.ac.uk, a.gartner@dundee.ac.uk)

    Using the forward genetic to investigate the DNA repair pathway in c.elegans

    • Manipulation of Nematode c.elegans (culture, isolate eggs, cross, backcross, freezing)
    • Mutagenesis, Irradiation ionic, screening, gene mapping
    • PCR, DNA preparation, enzymatic digestion, plasmid preparation, sequencing analysis, microinjection, fluorescence microscopy

  • Chronodrive - Préparateur de commandes, Livraison

    CROIX 2011 - 2012

  • Carrefour - Mise en rayon, Hôte de caisse vente traditionnelle

    Massy 2010 - 2011

  • Okaidi - Préparateur de commandes

    Roubaix 2009 - 2009

Formations

Réseau

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