Paris2007 - 2010Infection and epidemiology departement.
Anti-viral immunity, biotherapy and vaccine unit.
Established the apoptosis mechanisms by wich aNK cells induce the apoptosis of immature dendritic cells.
Studied the impact of HIV infection of dendritic cells on aNK-induced cell death.
Scientific articles publication and two patents.
Techniques used/optimized:
- Planning and carrying out experimental in vitro tests
- Flow cytometry
- Fluorescence 3D-confocal microscopy imaging of NK-DC crosstalk
- Real-time 3D video-microscopy of NK-DC cocultures
- Optimisation of transfection of primary dendritic cells
- siRNA technology of gene silencing
- Gene array analysis of apoptotic genes
- ELISA assays
- Inhibition assays
- scientific collaborations
- writing scientific articles
- Poster and oral presentations at national and international scientific conferences
Institut Pasteur
- Internship for M.Sc.
Paris2006 - 2007Internship for M.Sc. graduation.
Studied the impact of an HIV infection on the NK-DC crosstalk.
Experience:
- Cell culture of primary immune cells (NK cells, monocyte differentiation toward dendritic cells).
- HIV infection of dendritic cells and HIV replication monitoring (p24 staining, p24 ELISA and proviral DNA quantification).
- Flow cytometry: Intracellular/extracellular stainings; apoptosis assays (7-AAD). Cell quest and Flowjo softwares for aquisition and analysis respectively.
- ELISA assays.
Formations
Université Paris 7 Denis Diderot (Paris)
Paris2007 - 2010Imunologie
Institut Pasteur - Immunité anti-virale biothérapie et vaccins
