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Merial
- R&D Project Planner (professional training contract)
Lyon
2015 - maintenant
Planning and coordination of new product's development projects
Planning and coordination of transversal projects for continual improvement of project management process
Team Manager : Laure GAHINET
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Sanofi Pasteur
- Stagiaire
Lyon
2015 - 2015
Research & Development intership (Master 2)
Development of a method to quantify minor mutant viral populations in a live attenuated virus vaccine by high throughput sequencing
Team Manager : Eric SARCEY
Trainee's supervisor : Eric SARCEY & Audrey CHAREYRE
Skills :
- Molecular Biology : RNA Extraction, PCR, High Throughput Sequencing
- Bioinformatics : Primer design, Sequencing data analysis
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Visionari
- Patent Shaker Booster
2014 - 2014
Innovative R&D project development in biotechnology : Find an innovative idea for medicine, imagine the start-up which will develop the idea : preparation of business plan, planning development and production for 3 years.
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Visionari
- Patent Shaker (First Edition) Laureate
2014 - 2014
Innovative R&D project development in biotechnology : Feasibility study of a start-up based on the utilization and promotion of a patent, provided by Inserm Transfert.
Project : novICOS (http://novicos.makesit.net/)
Scientific analysis, Funding research, Business plan, Oral presentation in presence of expert jury
http://www.unimes.fr/fr/util/actualites/generales/archives-actus.html
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NanoMedSyn, IBMM, University of Pharmacy, Montpellier (FRANCE)
- Stagiaire
2013 - 2014
Research trainee (Master 1)
Production and characterization of targeted drugs for lysosomal storage diseases and some emerging cancers
Team Manager : Dr Marcel GARCIA
Trainee’s supervisor : Dr Marcel GARCIA & Dr Ilaria BASILE
Skills :
- Molecular Biology : RNA extraction, PCR
- Cell Culture : Cell line culture
- Biochemistry : WesternBlot, Activity and affinity analyses, Enzyme internalisation test
- Bioinformatics : Image analysis (Image J)
- Animal experiment
- Introduction to management and funding plans
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University of Queensland-Translational Research Institute-Diamantina Institute, Brisbane (AUSTRALIA)
- Stagiaire
2013 - 2013
Research trainee (BAC+3)
The expression of HPV16-E7 oncoprotein in skin enhances the production of TSLP-1, leading to a high Mast cells infiltration and Th2 microenvironment.
Team Manager : Dr Ian FRAZER
Trainee’s supervisor : Dr Anne-Sophie BERGOT
Skills :
- Molecular Biology : RNA extraction and quantification, RT-PCR
- Cell Culture : Cytometry, Primary culture
- Immunology/Virology : in vivo mice model expressing HPV16-E7 protein
- Bioinformatics : Cytometry (FlowJo), Image analysis (Image J), PCR (Applied BioSystems) , Statistics (GraphPad Prism)
- Animal experiment
Publication :
HPV16 E7 expression in skin induces TSLP secretion, type 2 ILC infiltration and atopic dermatitis-like lesions
Anne-Sophie Bergot, Nastasia Monnet, Son Le Tran, Deepak Mittal, Jane Al-Kouba, Raymond J Steptoe, Michele A Grimbaldeston, Ian H Frazer and James W Wells
Immunology and Cell Biology , (20 January 2015)
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ESTBB ((School of Biology-Biochemistry-Biotechnologies)
- Technical and financial feasibility study of medical analysis laboratory
2012 - 2013
Development of medical analysis laboratory in community health centres in Congo Brazzaville : prepare strategy, business plan and communication campaign according to high quality (quality manual writing, ISO 9001) and health & security.
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Centre de Recherche en Cancérologie de Lyon
- Stagiaire
2012 - 2012
Research trainee (BAC+2)
Study of the RNU2 CNV (Copy Number Variation) stability, located close to BRCA1 breast cancer predisposition gene
Team Manager : Dr Sylvie MAZOYER
Trainee’s supervisor : PhD Chloé TESSEREAU
Skills :
- Genetic : Molecular combing
- Molecular Biology : DNA extraction and quantification, quantitative RT-PCR, Fluorescence in situ hybridization (FISH)
- Cell Culture : Cell line culture
- Bioinformatics : Image analysis (Image J, JMeasure), PCR (Applied BioSystems)
Publication :
Direct visualization of the highly polymorphic RNU2 locus in proximity to the BRCA1 gene.
Tessereau C, Buisson M, Monnet N, Imbert M, Barjhoux L, Schluth-Bolard C, Sanlaville D, Conseiller E, Ceppi M, Sinilnikova OM, Mazoyer S.
PLoS One. 2013 Oct 11;8(10):e76054
Estimation of the RNU2 macrosatellite mutation rate by BRCA1 mutation tracing.
Tessereau C, Lesecque Y, Monnet N, Buisson M, Barjhoux L, Léoné M, Feng B, Goldgar DE, Sinilnikova OM, Mousset S, Duret L, Mazoyer S.
Nucleic Acids Res. 2014 Aug;42(14):9121-30