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Pauline PEUZIAT

RENNES

En résumé

Biotechnology engineer, 5 years of experience, actively seeking a position in Rennes area. Highly motivated, keen on learning new technologies, and open minded.

Mes compétences :
Microbiologie
Immunologie
Biotechnologies
Culture cellulaire
Optimisation des process

Entreprises

  • NG Biotech - R&D Engineer in Biotechnology

    2017 - 2018 (Fixed-term contract : 14 months)

    Development and optimization of lateral flow immunochromatographic tests for medial, veterinary and food.

    - Feasibility studies : Selection of raw materials and the design of the test, Use of different detection techniques (colloidal gold, cellulose beads, latex beads),
    - Products optimization,
    - Performance validation, Stability studies (accelerated, real time, in-use, etc),
    - Transfer to production
    - Reports writing, participation to the creation of technical files.

  • Microbs - R&D Engineer in microbiology

    2016 - 2017 (Fixed-term contract : 12 months)

    Development of an innovative and ultra-rapid technology for the microbiological quality assessment in food, pharmaceuticals and cosmetics.
    In charge of the development of one technological part for the technology design. Achievement of the feasibility study and start of the development phase.

    - Evaluation and selection of suppliers
    - Project management, report writing, implementation of action plan
    - Microbiology, Flow cytometry, Microscopy, Filtration

  • ImPACcell - Biosit - R&D Engineer

    2015 - 2016 (Fixed-term contract : 8 months)

    ImPACcell offers cellular content analysis services (HCS / HCA) to detect, analyze and quantify biological events and biomarkers such as proliferation and motility, cytoskeleton organization,cell cycle and apoptosis in various cell types and in small organizations. It also offers cytotoxicity and genotoxicity assays in order to determine the effects of acute and chronic exposure to either individual compounds or mixtures.

    - Development of new biological tests for the evaluation of molecule effect, on in vitro models
    - Cell culture (cell lines and primary cells, monolayer and 3D models)
    - Immunofluorescence, microscopy
    - Scientific watch

  • Inserm U1087 - Research Engineer

    2013 - 2015 (Fixed-term contract : 24 months)

    Project : Development and characterization of cationic liposomes, as non-viral vectors, for intracellular delivery of biological macromolecules (DNA, siRNA, mRNA, peptides, proteins)

    - Optimization of cationic liposomes for in vitro cell transfection : cell culture (cell lines and primary cells), transient transfection, microscopy, flow cytometry.
    - Characterization of humoral and cellular response after in vivo injection with synthetic vectors : ELISA and ELISpot.
    - Physicochemical characterization of vectors.

  • UCB - Intern in cell culture process development

    COLOMBES 2012 - 2013 Cell culture process development

    Project : Development and optimization of a fed-batch production process for monoclonal antibody by CHO Cells and cell line stability study.

    -Process development for monoclonal antibody production by mammalian cells (CHO cells) using 2L and 10L stirred tank bioreactors.
    -Feeding strategy and seeding density optimization.
    -Cell line stability study (in shake flasks and 2L bioreactors)
    -Design of experiment, statistical analyses (JMP®).
    -Process development documentation writing (sop, batch record, report)

  • Polymaris Biotechnology - Intern in microbiology

    2011 - 2011 Project : Isolation and characterization of marine bacterial strains for exopolysaccharide production.

    -Biological samplings
    -Isolation of marine bacterial strains by spreadings and enrichment methods
    -Characterization of strains producing exopolysaccharides (Gram staining, sporulation tests)
    -Fermentation monitoring at small and large scall

Formations

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