Margaux Sarah DELALOIX

En résumé

Entreprises

• Merck - Process validation scientist, expert - USP

  Lyon 2019 - maintenant Project scientist / Process validation Expert, USP - Biotech Process Sciences (BPS) department
  Merck Group - temporary position until December 31th, 2019
  Affected to multiple projects at different stages, act as a BPS validation team member and USP expert
  • Contact point for expertise to Manufacturing, USP, DSP, Analytics, support, QC and QA department
  • In charge of unexpected events investigations (deviations, investigations on root cause)
  • Responsible for validation deliverables:
  o Collect data about cell culture process performances indicators and product quality
  o Analyze and interpret in order to integrate in reports
  o Write/edit (author or co-author), review, approve reports on Upstream process development/validation: stability study, development process, model suitability/qualification, range confirmation, assessment reports on small-scale and large-scale experiments

• Université de Lausanne - Postdoctoral scientist

  2017 - 2017 Scientist, post-doctoral - research on the improvement of new biotech methods at lab-scale model
  Lausanne University (UNIL-EPFL), center for Biotechnology
  In charge of improvement of the recombinant cell lines selection method based on vitamin metabolism
  • Writing lab-book, reports and protocols
  • Design and execution of experimentations:
  o Generation of recombinant mouse stem cells and CHO cell lines producing therapeutics or monoclonal antibody
  o Screening of optimal cell culture conditions in minimal vitamin medium by studying cell growth and viability assays

• CHUV, Laboratoire R&D du service IAL - Ph.D Student

  2013 - 2016 Research scientist, PhD student - academic research and clinic trial lab on mucosal immunity and infectious diseases
  Lausanne University State Hospital (CHUV), Division of Immunology and Allergy - R&D laboratory
  In charge of a research project: Retrotransport of secretory IgA by the human Transferrin receptor through intestinal epithelial cells
  • Writing lab-book, reports, protocols, scientific article and thesis manuscript
  • Analysis of results by bioinformatics and statistical tools
  • Design, development, and execution of experimentations:
  o Development of in vitro models for study of protein-protein interactions (adherent and polarized Caco-2 cells)
  o Development of immuno-assays, multi-color flow cytometry and histological analysis
  o Establishment of CHO cell lines overexpressing a cell surface protein (transfection and clones expansion/selection)

• Pierre Fabre - Junior research scientist

  Castres 2013 - 2013 Junior research scientist, master thesis - Pierre Fabre, Dermo-cosmetic branch, cell-pharmacology team
  Project: Development of Atopic Dermatitis (AD) in vitro model, research of new biomarkers
  • Efficiency and toxicology assessment of new active substances (gene expression and cell phenotype analyses)
  • Preparation of human RNA samples for DNA micro-arrays and analysis of DNA micros-arrays on Ingenuity

• Inserm, équipe EA3175 Avenir, Limoges, CNR du Cytomégalovirus - Lab technician

  PARIS 13 2011 - 2011 Lab. Technician, internship position - University hospital of Limoges, UMR CHU - INSERM - UniLim 1092
  Project: Development of in vitro expression model of Cytomegalovirus terminases
  • Construction of recombinant plasmids/vectors expressing viral proteins
  • Transfection of recombinant plasmids/vectors in mammalian cell lines

• La Poste - Student job in Post Office

  Paris-15E-Arrondissement 2009 - 2013 2009 to 2013, during school holidays and weekend in Limoges and Paris
  Customer relationship, sales and advice (in mail or article shipment, cell phone products and services, international money transfers) and bank operations/services

• INRA Tours, UR IASP, Equipe Immunologie aviaire - Lab technician

  2009 - 2009 Lab. Technician, internship position - INRA - UMR 1282 ISP - team VIA
  Project: Purification and characterization of avian chemokines
  • Purification of recombinant avian proteins by chromatography from bacterial clones
  • Characterization of recombinant avian proteins by immunological and bioanalytical assays

Formations

• Université De Lausanne (UNIL) (Lausanne)

  Lausanne 2013 - 2016 PhD student

• Université Toulouse 3 Paul Sabatier

  Toulouse 2012 - 2013 Master 2 Professionnel
  
  Course in immunology, vectorology, genetic, molecular biology, gene therapies and vaccinology.
  Drafting and oral presentation of a research paper: Development of a vaccine against Chikungunya infection (literature review, identification of the most antigenic viral protein and anti Chik immune response and evaluation of the best strategy for producing the antigen)

• Université Paris 7 Denis Diderot

  Paris 2011 - 2012 Master 1 IMVI
  
  Course in immunology, virology, pathogenic guest relations, Immunophysiopathology at Medecine school in the University Paris Descartes, pharmacogenetics, biochemistry, molecular biology and physiology / bacterial genetics
  group work on scientific papers for oral presentations
  practical work in immunology, molecular biology and virology

• Université Des Sciences De La Rochelle

  La Rochelle 2009 - 2011 Licence
  
  degree in biology biochemistry (second and third year )
  
  courses in biochemistry, spectral analysis, physiology, metabolism, immunology, virology, structural biochemistry, protein characterization, molecular biology, cell biology.

• IUT Génie Biologique

  La Rochelle 2007 - 2009 DUT Génie Biologique
  
  Courses in food technology, chemistry, biochemistry, microbiology

• Lycée Raoul Dautry

  Limoges 2005 - 2007 Baccalauréat technologique

Réseau

• Anaïs DOMERGUE

• Aurore NUGUES

• Guilain COUTTENIER

• Julie MARIE

• Laurent GRENIER

• Magalie MICHEE-COSPOLITE

• Natacha BOUTAULT

• Olivier ROUX