Menu

Mikidache MADI

CERGY ST CHRISTOPHE

En résumé

Domaines de Compétence:
Clonage, Séquençage, Mutagenèse dirigée, Extraction d’ADN et d’ARN
Production et Purification de protéines recombinantes
Culture cellulaire, Transfert de gène (Electroporation, Lipofection)
Micro-injection de cellules ES
PCR en temps réel (TaqMan et SYBR Green)

Logiciels : MS Project, Word, Excel, Power Point
Langues:
Anglais : pratique professionnelle scientifique
Allemand : niveau baccalauréat

Mes compétences :
Cell culture
Drug discovery
Western Blotting
HPLC
Cloning
Scientific paper writing
Recombinant protein
Scientific research
ELISA

Entreprises

  • The Pirbright Institute - Research Assistant

    2009 - maintenant - Working in biosecurity level 3 containment laboratory under ISO 17025 regulations.
    - Responsible for molecular laboratory management.
    - Molecular diagnostic and characterisation of livestock disease such as Foot-and-Mouth Disease and Vesicular Stomatitis Disease Virus by virus isolation, ELISA, real-time RT-PCR and sequencing.
    - Evaluation of pen-side technologies.
    - Protocol development and improvement.
    - Analyse and presentation of research results during meeting and conferences.
    - Participate in writing research papers.
    - Mentoring one year placement sandwich students.
    - Assist in training delegates, staff and PhD students in molecular biology and serological techniques
    - Actively seek to maintain and improve the institute health and safety/biosecurity standards.
  • Pfizer - Cellular and Molecular Biologist

    Paris 2008 - 2008 - Application of knowledge in the design and execution of experimental work (Primer design, Cloning, Q-PCR, Western Blotting).
    - Strengthening the gene expression profiling capabilities of the current team.
    - Proficiency in a range of increasingly complex cloning techniques (subcloning concatamer gene).
    - Critical appraisal and presentation of the results to molecular pharmacology team.
    - Use of COSHH and generic risk assessments and Job Based Hazard Assessments.
    - Working independently and in GCPCR’s and ion channel team on multiple subjects.
  • Hopital Beaujon - Phase I Clinical Research

    2007 - 2008
  • INSERM - Cellular and Molecular Biologist

    PARIS 13 2006 - 2007 Biotherapeutic team involveg in Genetherapy:
    - Protocol design, managing of experimental schedule.
    - DNA,RNA and protein extraction.
    - ELISA, Enzymatic activity assay, Protein dosage and HPLC.
    - Reverse Transcriptase-PCR and Q-PCR in 384 wells plate.
    - Genotyping of Adrenoleucodystrophy patients (through SNP sequencing).
    - Analysis, synthesis, and presentation of the results to peers.
  • Bio-rad - Cellular and Molecular Biologist Internship

    Marnes La Coquette 2005 - 2005 “Validation of a HPV DNA extraction kit by real time PCR”
    - Protocols elaboration, experiments schedule management
    - Routine Cell culture,HPV DNA Extraction, Q-PCR
    - Results analysis, Reporting and presentation to peers

Formations

Réseau

Annuaire des membres :