LAUSANNE
2012 October to date - Postdoctoral position in immunology. Group of Pr. Michel Gilliet. Lausanne - Switzerland
2012 January - PhD in Immunology - University of Paris XI
2008 2011 - PhD student in Immunology. University Paris XI. Host laboratory: Unit 1012 INSERM, French National Institute of Health and Research Medical
2007 - Master Immunology
2003 to 2006 - IUP Genomic and proteomic of Lille 1
PhD project : Causes and consequences of type I interferon activation and epigenetic regulation in autoimmune diseases (especially in Sjögren’s Syndrome)
Publications : Author - First
Upregulation of membrane-bound CD40L on CD4+ T cells in women with primary Sjögren s syndrome.
Gestermann N et al. Scand J Immunol. 2013 Oct 5.
Methylation profile of the promoter region of IRF5 in primary Sjögren's syndrome.
Gestermann N et al. Eur Cytokine Netw. 2012 Oct-Dec;23(4):166-72.
STAT4 is a confirmed genetic risk factor for Sjögren s syndrome and could be involved in type 1 interferon pathway signaling.
Gestermann N et al. Genes Immun. 2010 Jul;11(5):432-8
The CGGGG insertion/deletion polymorphism of the IRF5 promoter is a strong risk factor for primary Sjögren s syndrome. Gestermann N et al. Arthritis Rheum. 2009 Jul;60(7):1991-7.
Effect of methotrexate and anti-TNF on Epstein-Barr virus T-cell response and viral load in patients with rheumatoid arthritis or spondylarthropathies. Gestermann N et al. Arthritis Res Ther. 2009; 11(3):R77.
Others publications :
Interleukin-7 influences FOXP3+CD4+ regulatory T cells peripheral homeostasis.
Simonetta F, Gestermann N, Martinet KZ, Boniotto M, Tissières P, Seddon B, Bourgeois C. PLoS One. 2012;7(5):e36596. doi: 10.1371/journal.pone.0036596
Functional study of TNF-a promoter polymorphisms, literature review and meta-analysis. Mekinian A, Tamouza R, Pavy S, Gestermann N, Ittah I, Mariette X, Miceli-Richard C. Eur. Cytokine Netw.2011 Jun 1;22(2):88-102
Decreased B cell activating factor receptor expression on peripheral lymphocytes associated with increased disease activity in primary Sjögren s Syndrome and systemic lupus erythematosus. Sellam J, Miceli-Richard C, Gottenberg JE, Ittah M, Lavie F, Lacabaratz C, Gestermann N, Proust A, Lambotte O, Mariette X. Ann Rheum Dis. 2007 Jun;66(6):790-7.
Expertise :
Molecular biology:
- RNA and DNA extraction, RT-PCR, PCR, qPCR (Light Cycler and Biorad)
- Pyrosequencing (Methylation analysis, Biotage, Qiagen)
Cellular biology:
- Flow cytometry (Canto BD, Calibur): membrane staining, intra-cellular staining, nuclear staining.
- Cells separation (Magnetic beads, MoFlo cells sorting)
- Human cells culture : CD4+ T cells, Monocytes, B Lymphocytes, Plasmacytoïd dendritic cells, Myeloid dendritic cells
- Salivary gland epithelial cells from biopsy
- Cells line: K562, U937, PRI97, Molt-4, Jurkat
- Western Blot,Elispot, Elisa
- Antibodies cloning from EBV-B cells immortalized
- Confocal
Mes compétences :
Confocal analysis
Immunology
Génétique
Culture cellulaire
