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Florent COLLAS

WAGENIGEN

En résumé

Mes compétences :
Visual Basic
Scilab
Microsoft Office
Fermentation avec micro-organismes anaerobies
Biotechnologies blanches
Fermentation
Biotechnologies
Biologie moléculaire
Microbiologie

Entreprises

  • Food and Biobased research (Wageningen University research) - Ingenieur de recherche

    2014 - maintenant

  • IFPEN - Ingenieur biology moleculaire

    2012 - 2013 Mise en place de l'activité génétique dédiée au microoroganismes du genre Clostridium.
    Developpement d'outils génétiques dédiés aux Clostridum.
    Formation et supervision de deux techniciens

  • Food and Biobased research (Wageningen University Research) - Doctorant (en collaboration avec l'IFPEN)

    2009 - 2011

  • IFPen - Lyon - Doctorant

    2008 - 2011 Over the past decades, the IBE fermentation (isopropanol, butanol and ethanol) has received a renew interest for the production of fuels or biochemicals from renewable materials. In the present study, we have investigated the IBE fermentation using the natural producer C. beijerinckii NRRL B593 and genetically-modified strains of Clostridium acetobutylicum ATCC 824. In batch culture, C. beijerinckii NRRL B593 was found to excrete 13.2 g/L IBE of which 4.5 g/L was isopropanol. To increase IBE production, the gene coding the secondary alcohol dehydrogenase (s-Adh) of C beijerinckii NRRL B593 and different combinations of genes coding for enzymes active in acetoacetyl-CoA to acetone conversion i.e. acetoacetate decarboxylase (adc) and acetoacetyl-CoA: acetate/butyrate: CoA transferase subunits A and B (ctfA and ctfB) were expressed in the ABE (acetone, butanol ethanol) producer C. acetobutylicum ATCC 824. Results showed that the overexpression of the ctfA and ctfB genes significantly increased both speed and extent of the IBE production while the overexpression of the adc gene had only a little effect. In batch culture, the best mutant (expressing adh, ctfA, ctfB and adc) produced 24.4 g/L IBE (of which 8.8 g/L was isopropanol) and displayed an IBE productivity of 0.7 g/L h. Cultivated in continuous mode at the dilution rate of 0.1 h-1, IBE productivity was increased to 1.7 g/L h IBE. As the IBE mix has been considered as a valuable fuel additive, the transformants obtained are a real step forward towards the development of an industrial IBE process for biofuel production.

  • AUCHAN - "Conseiller vente" (Emploi étudiant)

    Villeneuve-d'Ascq 2004 - 2008

Formations

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