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Amandine ONGARO

Boulogne-Billancourt

En résumé

Mes compétences :
Antibody
ELISA
Cell Culture
Biotechnology
sequence analysis
antibody analysis
Microsoft Office
DNA cloning

Entreprises

  • Roche - Senior Associate in Molecular Biology

    Boulogne-Billancourt 2014 - maintenant Molecular biology specialist for three portfolio projects
    - design and generation of plasmids for antibody expression in a large range of complex formats
    - DNA and TOPO cloning, PCR, multi-site directed mutagenesis, DNA preparation from mini to giga scale, sequence analysis

    Molecular biology specialist for one functional excellence project
    - Gibson assembly, Southern Blot

    Evaluation of a new device for DNA electrophoresis (TRAce system)

    Exchange stay period in the Phage Display team
    - from phage library generation to binders selection
    - Dotblot, ELISA, introduction to Tecan system

    Scientific presentations and posters, participation to Journal Club sessions


    Purchase requester for the molecular biology team (use of SAP, commercial meetings, set up of consignment stock)

  • Roche - Research Associate

    Boulogne-Billancourt 2012 - 2014

  • NovImmune - Intern in the Protein Generation unit

    2011 - 2012 Cliquez pour modifier la description du posteGeneration of vectors and development of purification for nouvel bispecific antibody formats
    - molecular biology: PCR, DNA cloning, mutagenesis
    - cell culture: transfection and culture of mammalian cells
    - purification: batch system with different affinity resins
    - antibody analysis: protein gels, ELISA and basics in Biacore

  • Eurotest - Intern in the Research and Development department

    2010 - 2010 Development of a melanin assay method, study conducted in vitro on melanocytes and keratinocytes in culture
    - cell culture: culture and co-culture of mammalian cells
    - analytical methods: assay, cytotoxicity

Formations

  • ENSTBB (Ecole Nationale Supérieure De Technologie Des Biomolécules De Bordeaux) (Bordeaux)

    Bordeaux 2009 - 2012 Master's degree in Biotechnology

    Institute of Biotechnology specialized in production, purification and characterization of biomolecules
    http://www.enstbb.ipb.fr/en

  • Lycée Carnot

    Dijon 2007 - 2009

Réseau

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