Amandine ONGARO

En résumé

Entreprises

• Roche - Senior Associate in Molecular Biology

  Boulogne-Billancourt 2014 - maintenant Molecular biology specialist for three portfolio projects
  - design and generation of plasmids for antibody expression in a large range of complex formats
  - DNA and TOPO cloning, PCR, multi-site directed mutagenesis, DNA preparation from mini to giga scale, sequence analysis
  
  Molecular biology specialist for one functional excellence project
  - Gibson assembly, Southern Blot
  
  Evaluation of a new device for DNA electrophoresis (TRAce system)
  
  Exchange stay period in the Phage Display team
  - from phage library generation to binders selection
  - Dotblot, ELISA, introduction to Tecan system
  
  Scientific presentations and posters, participation to Journal Club sessions
  
  
  Purchase requester for the molecular biology team (use of SAP, commercial meetings, set up of consignment stock)
  

• Roche - Research Associate

  Boulogne-Billancourt 2012 - 2014

• NovImmune - Intern in the Protein Generation unit

  2011 - 2012 Cliquez pour modifier la description du posteGeneration of vectors and development of purification for nouvel bispecific antibody formats
  - molecular biology: PCR, DNA cloning, mutagenesis
  - cell culture: transfection and culture of mammalian cells
  - purification: batch system with different affinity resins
  - antibody analysis: protein gels, ELISA and basics in Biacore

• Eurotest - Intern in the Research and Development department

  2010 - 2010 Development of a melanin assay method, study conducted in vitro on melanocytes and keratinocytes in culture
  - cell culture: culture and co-culture of mammalian cells
  - analytical methods: assay, cytotoxicity

Formations

• ENSTBB (Ecole Nationale Supérieure De Technologie Des Biomolécules De Bordeaux) (Bordeaux)

  Bordeaux 2009 - 2012 Master's degree in Biotechnology
  
  Institute of Biotechnology specialized in production, purification and characterization of biomolecules
  http://www.enstbb.ipb.fr/en

• Lycée Carnot

  Dijon 2007 - 2009

Réseau

• Antonin LOOF

• Delphine PORTES

• Esther ATTEBI

• Fabrice CATTANT

• Florent FORDOXEL

• Margaux JAY

• Maxime VERGNE

• Pierre VATIER

• Raphael PEUCH

• Stéphanie PARMENTIER